Because we were unable to validate our primers in our original gel electrophoresis, we are unable to be positive if it was something wrong with this specific PCR or if the primers do not anneal as expected. We verified our primer length with the programs, so it could be possible that it bound to a different spot than expected.
It is a possibility that it could impact our results. The varying length could be due to the fact that the primers annealed to a different area than expected. Although, the fact that both of the PCRs resulted in bands at the same location helps to confirm that the different bonding probably does not impact the varying expression.
It is a possibility that it could impact our results. The varying length could be due to the fact that the primers annealed to a different area than expected. Although, the fact that both of the PCRs resulted in bands at the same location helps to confirm that the different bonding probably does not impact the varying expression.
It is a possibility that it could impact our results. The varying length could be due to the fact that the primers annealed to a different area than expected. Although, the fact that both of the PCRs resulted in bands at the same location helps to confirm that the different bonding probably does not impact the varying expression.
It is a possibility that it could impact our results. The varying length could be due to the fact that the primers annealed to a different area than expected. Although, the fact that both of the PCRs resulted in bands at the same location helps to confirm that the different bonding probably does not impact the varying expression.
curesymposium.org 
Why do you think the bands for the designed primers were shorter than expected?
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Because we were unable to validate our primers in our original gel electrophoresis, we are unable to be positive if it was something wrong with this specific PCR or if the primers do not anneal as expected. We verified our primer length with the programs, so it could be possible that it bound to a different spot than expected.
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Does the fact that the bands were shorter than expected impact the efficacy of the result?
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It is a possibility that it could impact our results. The varying length could be due to the fact that the primers annealed to a different area than expected. Although, the fact that both of the PCRs resulted in bands at the same location helps to confirm that the different bonding probably does not impact the varying expression.
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Does the fact that bands were shorter than expected impact the efficacy of you results?
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It is a possibility that it could impact our results. The varying length could be due to the fact that the primers annealed to a different area than expected. Although, the fact that both of the PCRs resulted in bands at the same location helps to confirm that the different bonding probably does not impact the varying expression.
LikeLike
It is a possibility that it could impact our results. The varying length could be due to the fact that the primers annealed to a different area than expected. Although, the fact that both of the PCRs resulted in bands at the same location helps to confirm that the different bonding probably does not impact the varying expression.
LikeLike
Does the fact that the RNA was shorter than expected impact the efficacy of your results?
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It is a possibility that it could impact our results. The varying length could be due to the fact that the primers annealed to a different area than expected. Although, the fact that both of the PCRs resulted in bands at the same location helps to confirm that the different bonding probably does not impact the varying expression.
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