11 thoughts on “C37 – Bivrell

  1. If the lanes varied in its brightness in regards to the gene regulation electrophoresis, what would that suggest about your data?

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    1. Hi! the brightness of the bands referred to the amount of expression of each gene. A brighter band would mean that our gene expression was up-regulated and a dimmer band would mean that there was less of our gene expressed after experimentation.

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  2. Hello! Thanks for presenting your poster!
    So my question is, what are the specific methodologies are you looking to do after your results are inconclusive of whether this gene plays a role in DNA damage repair?

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    1. Hi! We would definitely be looking at recreating our final batch of PCRs, specifically our cDNA PCRs from the extracted mRNA in our gene specific damaged and undamaged lanes. If this was effected in any way we would see our current results and any changes would most likely to be able to explain why we got the results that we did.

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  3. Hi, your presentation was excellent and very precise. Are the water lanes in the gel electrophoresis negative controls? If not what do they indicate? Thank you!

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    1. Hi! yes the water in the gel lanes are negative controls. If there were any results in the water lanes that would most likely be due to contamination.

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    1. Hi! Rdn2 was most likely not expressed on the gene lanes due to improper cDNA accumulation in or before we created our final PCRs

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  4. Great Job!
    You mentioned alternative methodologies or approaches to finding how RDN2 gene plays a role in DNA repair. Could you mention one methodology you would use moving forward to explore this?

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  5. Great presentation! I was just wondering what hydroxyurea (what was used to induce the DNA damage to the cells) is exactly?

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