I think the “tDNA” you’re referring to is the UT DNA? If so, that is the untreated DNA (the DNA that is not treated with hydroxyurea to induce DNA damage) we used when evaluating whether or not our gene was expressed when double stranded DNA damage has occurred. The other DNA we used “HU DNA” was treated with hydroxyurea to induce damage. These two states were compared.
The spreadsheet is the quantitative analysis of the gel presented in the poster. The purpose was to show numerically if there were any significant difference in the gene expression between HU DNA and UT DNA between the controls and our gene Twi2.
Hello, I was wondering if you could you elaborate on what tDNA is and what the spreadsheet signifies in the results section?
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Hi Haley!
I think the “tDNA” you’re referring to is the UT DNA? If so, that is the untreated DNA (the DNA that is not treated with hydroxyurea to induce DNA damage) we used when evaluating whether or not our gene was expressed when double stranded DNA damage has occurred. The other DNA we used “HU DNA” was treated with hydroxyurea to induce damage. These two states were compared.
The spreadsheet is the quantitative analysis of the gel presented in the poster. The purpose was to show numerically if there were any significant difference in the gene expression between HU DNA and UT DNA between the controls and our gene Twi2.
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