I would redo the validation of the primers (figure 2) since the cDNA and gDNA got switched because the marker got rubbed off the tubes. Thank you for the question.
What would be the desired results for silencing the RRM2 gene in the future experimentation and what would they show in the context of the research question?
The failed results would be if the expression of +RT +HU 14-BGS was less than +RT -HU 14-BGS, which can be seen in figure 3. Failed results would mean that RRM2 would not be involved in the DNA damage repair response. Thank you for the question.
curesymposium.org 
What is something you would do differently if you had the chance to run the trial again?
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I would redo the validation of the primers (figure 2) since the cDNA and gDNA got switched because the marker got rubbed off the tubes. Thank you for the question.
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What would be the desired results for silencing the RRM2 gene in the future experimentation and what would they show in the context of the research question?
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The desired result would still be the confirmed role in DNA damage exists if the gene is silenced. Thank you.
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What would the results of lesser quality or failed results look like?
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The failed results would be if the expression of +RT +HU 14-BGS was less than +RT -HU 14-BGS, which can be seen in figure 3. Failed results would mean that RRM2 would not be involved in the DNA damage repair response. Thank you for the question.
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How would you determine the RRM2 gene structure?
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You would determine the structure by identifying the gene through a gene database using the gene name and/or symbol. Thank you.
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