Thank you! The Cas9 enzyme in CRISPR will be used to used to cut out the Twi8 gene and the phenotypic outcomes of this will determine the role of Twi8 gene normally.
Would researchers have to incorporate this specific Twi8 gene into the human genome to get the desired outcome, or is there a different gene involved in human DNA repair that can be modified to act like the Twi8 gene?
curesymposium.org 
How would immunohistochemistry and cellular fraction help locate the Twi8 protein functions and give insight on the structure of the protein?
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This would allow for the cells to be split open and marked for Twi8 to visualize where this protein functions.
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This would allow for the cell to be split and marked for Twi8 gene to see where the protein is functioning.
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What would you want to do with the information of the Twi8 protein if you have more insight on the function and structure?
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To learn more about how sRNA’s repair DNA and how this can be utilized for further therapies.
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Great Presentation! What are some internal and external factors that contribute to DNA damage?
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Thanks! Internal Factor could be replication stress and oxygen radicals. External Factors could be UV radiation and viral infection.
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Hello, great presentation! I was wondering how CRISPR is used for Gene Knockout and how the phenotype is determined through this?
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Thank you! The Cas9 enzyme in CRISPR will be used to used to cut out the Twi8 gene and the phenotypic outcomes of this will determine the role of Twi8 gene normally.
LikeLike
Would researchers have to incorporate this specific Twi8 gene into the human genome to get the desired outcome, or is there a different gene involved in human DNA repair that can be modified to act like the Twi8 gene?
LikeLike