We think that either the Rsp1 gene is not expressed or there was experimental error where either DNA or primers were not added in our gene specific lanes.
We were never positive that Rsp1 plays a role, but it was hypothesized because the protein is associated with sRNAs that are involved. We think the results could either be due to a lack of expression or experimental error in the RT-PCR
If DNA damage is not repaired, it could lead to cell death and mutations in DNA. Either of these issues could cause cells to form irregularly or in ways they’re not supposed to, which could lead to diseases or cancer cells forming. Hope that answers your question!
If Rsp1 is involved in the repair process, it means that this gene plays a role as sRNAs repair double strand breaks in the DNA. Researching further into this could lead to more information about how the DNA damage repair process works as a whole and the importance of Rsp1 in preventing disease and cancer cell formation.
curesymposium.org 
What do you think happened to not not get the results that you had anticipated?
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We think that either the Rsp1 gene is not expressed or there was experimental error where either DNA or primers were not added in our gene specific lanes.
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Why do you think your results differed from the hypothesis that Rsp1 plays a significant role in the process of DNA damage repair?
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We were never positive that Rsp1 plays a role, but it was hypothesized because the protein is associated with sRNAs that are involved. We think the results could either be due to a lack of expression or experimental error in the RT-PCR
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What are some real world applications to repairing DNA?
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If DNA damage is not repaired, it could lead to cell death and mutations in DNA. Either of these issues could cause cells to form irregularly or in ways they’re not supposed to, which could lead to diseases or cancer cells forming. Hope that answers your question!
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What does it mean in the bigger picture if Rsp1 is involved in the DNA damage repair process or not?
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If Rsp1 is involved in the repair process, it means that this gene plays a role as sRNAs repair double strand breaks in the DNA. Researching further into this could lead to more information about how the DNA damage repair process works as a whole and the importance of Rsp1 in preventing disease and cancer cell formation.
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How would you change your methods if you were to repeat this experiment?
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We would likely use better parameters when designing primers so that we could hopefully use our own when moving on to RT-PCR.
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