When DNA is damaged and if Rdn2 can help repair that damage, is it possible to manually give Rdn2 to the DNA as a form of treatment and help speed up the repair or would that just cause an overload of Rdn2 and result in consequences?
So RDN2 is a gene that when expressed, activates siRNA’s, and those SiRNA’s are what repair the DNA. We can’t exactly manually give RDN2 because it is a part of the DNA, but we can alter its expression and over express it so it produces more siRNA’s and that could repair the DNA faster.
If there were a correlation (there is, our experiment doesn’t show it, but others did) then we can move forward in stating that RDN2 is incremental in the DNA repair pathway and we can find a way to more highly express that gene so DNA damage is more quickly repaired when presented.
I loved that you fully explained the real world applications of your poster. I think it made it really engaging. How would you further test to get a more conclusive result?
We did end up doing our PCR and gel electrophoresis twice and we failed to get bands in our RDN2 gene lanes, but when we tested our primers, they seemed to anneal fine. Our primers could’ve been degraded somehow, but I would definitely redo our entire experiment. That way we can hopefully get some kind of result.
You start off by discussing ways that a cell can get damaged, such as UV, but I want to further understand how a simple UV ray can hurt our cells and ultimately cause problems such as adverse side effects.
Additionally, I see that in your future directions you state potentially repeating and expanding your project to account for any error. If there was error, what do you expect to change? How would this affect your results?
So, UV comes from the sun and are extremely small rays that can cut straight through DNA. This can result in double-stranded DNA breakages which siRNAs are sent to fix the double-stranded DNA breakages. DNA damage has the potential of causing cancer or other problems.
Pertaining to our actual results, I would redo our entire experiment because we don’t necessarily know what went wrong, so hopefully, in redoing our experiment, we would be able to see results in our RDN2 band and we would be able to conclude our hypothesis and answer our research question. “Is RDN2 more expressed during DNA damage?”
curesymposium.org 
When DNA is damaged and if Rdn2 can help repair that damage, is it possible to manually give Rdn2 to the DNA as a form of treatment and help speed up the repair or would that just cause an overload of Rdn2 and result in consequences?
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So RDN2 is a gene that when expressed, activates siRNA’s, and those SiRNA’s are what repair the DNA. We can’t exactly manually give RDN2 because it is a part of the DNA, but we can alter its expression and over express it so it produces more siRNA’s and that could repair the DNA faster.
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What is the difference between cDNA and gDNA?
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cDNA is the coding DNA while gDNA is the genomic DNA. So gDNA is the whole DNA code with both the introns and exons, while cDNA is only the exons.
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Great Job! If there were a correlation between the
RDN2 gene and the DNA repair pathway what do you think that would imply?
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If there were a correlation (there is, our experiment doesn’t show it, but others did) then we can move forward in stating that RDN2 is incremental in the DNA repair pathway and we can find a way to more highly express that gene so DNA damage is more quickly repaired when presented.
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I loved that you fully explained the real world applications of your poster. I think it made it really engaging. How would you further test to get a more conclusive result?
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We did end up doing our PCR and gel electrophoresis twice and we failed to get bands in our RDN2 gene lanes, but when we tested our primers, they seemed to anneal fine. Our primers could’ve been degraded somehow, but I would definitely redo our entire experiment. That way we can hopefully get some kind of result.
LikeLike
You start off by discussing ways that a cell can get damaged, such as UV, but I want to further understand how a simple UV ray can hurt our cells and ultimately cause problems such as adverse side effects.
Additionally, I see that in your future directions you state potentially repeating and expanding your project to account for any error. If there was error, what do you expect to change? How would this affect your results?
Thanks, and great presentation.
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So, UV comes from the sun and are extremely small rays that can cut straight through DNA. This can result in double-stranded DNA breakages which siRNAs are sent to fix the double-stranded DNA breakages. DNA damage has the potential of causing cancer or other problems.
Pertaining to our actual results, I would redo our entire experiment because we don’t necessarily know what went wrong, so hopefully, in redoing our experiment, we would be able to see results in our RDN2 band and we would be able to conclude our hypothesis and answer our research question. “Is RDN2 more expressed during DNA damage?”
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