Cells use genes to transcribe and translate proteins that are essential to cell functioning. If the genes are damaged, the cell will most likely repair these genes. The repair process is not always exact, and it can introduce a mutation into the cell’s genome. If this mutation is part of an important gene, the proteins that are translated from this gene could not function properly. This could lead to cell death, or mutations that cause the cell to divide rapidly, causing cancer. Therefore, being able to successfully repair DNA damage is essential to cell survival, and targeting these pathways in defective cells can lead to effective therapies. This is because cells need to repair DNA damage constantly to survive. For example, if you block a pathway of repair in cancer cells, that can lead to the death of cancer cells in a patient, and thus be an effective therapy.
Hydroxyurea was the agent used to induce DNA damage, and we used it in our model organism to test if the expression of our gene increased after inducing DNA damage. This could lead us to conclude if it played a role in DNA repair.
No, there was very little data on this gene. However, it showed similar expression patterns to Rad51, which is a gene known to play a role in DNA repair.
I believe that the designed primers annealed to another part of our gene in our model organism. We saw faint bands at the incorrect length, so it could have been that our designed primer annealed to a different part of the organisms genome.
curesymposium.org 
What was the role of hydroxyurea?
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Hydroxyurea is the agent that we used to induce DNA damage!
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What similarity in expression of the Cory gene to Rad51 enlightened you to test and experiment the Cory gene?
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Both genes showed two peaks of expression during conjugation, at around the same times.
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Hi! How exactly does DNA damage result in these sicknesses and diseases?
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Cells use genes to transcribe and translate proteins that are essential to cell functioning. If the genes are damaged, the cell will most likely repair these genes. The repair process is not always exact, and it can introduce a mutation into the cell’s genome. If this mutation is part of an important gene, the proteins that are translated from this gene could not function properly. This could lead to cell death, or mutations that cause the cell to divide rapidly, causing cancer. Therefore, being able to successfully repair DNA damage is essential to cell survival, and targeting these pathways in defective cells can lead to effective therapies. This is because cells need to repair DNA damage constantly to survive. For example, if you block a pathway of repair in cancer cells, that can lead to the death of cancer cells in a patient, and thus be an effective therapy.
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Why did you choose hydroxyurea and what was its role in the experiment?
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Hydroxyurea was the agent used to induce DNA damage, and we used it in our model organism to test if the expression of our gene increased after inducing DNA damage. This could lead us to conclude if it played a role in DNA repair.
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Why did you decide to study Cory Gene? Is there previous data to suggest this gene may be involved?
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No, there was very little data on this gene. However, it showed similar expression patterns to Rad51, which is a gene known to play a role in DNA repair.
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What do you assume is the reason that the design primers did not anneal to the target sequence?
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I believe that the designed primers annealed to another part of our gene in our model organism. We saw faint bands at the incorrect length, so it could have been that our designed primer annealed to a different part of the organisms genome.
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