So when its upregulated, it means the gene is being transcribed more as the cell needs more of the resulting protein in the cell.
Conversely, if it is downregulated, then less of the resulting protein is needed in the cell so smaller amounts of the gene is transcribed.
In this experiment, we are inducing DNA damage so if our gene is involved in DNA repair, the cell would need more of the protein our gene codes for and thus it will be upregulated.
Can the identification of the RDN2 gene as necessary to repair DNA damage help with other health issues besides cancer, or can it help with industries outside of the healthcare field?
It could have to potential to play a role in any biological process/treatment that involves the repair and maintenance of DNA. This might include anti-aging treatments or GMO research.
Hi! Your presentation was great! Is there a way to change the quantity of the RDN2 gene if it presents in small quantities when doing research? Kind of like phages how you are able to genetically engineer them to become lytic after originally being temperate?
You might be able to use a higher cell count in your initial sample however this may have negative effects on your controls. Ideally we would just use a more sensitive technique such as rt-qPCR which would provide a much more quantitative result.
Other methods include quantitative PCR which would allow us to quantitatively compare the regulation of the gene before and after DNA damage has been induced.
cDNA (copy DNA) is created using mRNA. Single stranded mRNA is reverse transcribed into double stranded DNA creating a strand of DNA that does not contain any introns.
In the Future Directions portion of your poster you mention that there are alternative approaches to obtaining data about the RDN2 gene. Would you mind elaborating on what the procedure or general basis of these alternative approaches are when compared to your procedure?
Hi! Your presentation as really good! Are there other experiments that you would need to do to confirm your results.
Prior to you experiment, do you know if your specific gene is upregulated, down regulated, etc.
Finally, can you explain the role of hydroxyurea.
Thank you so much 🙂
Hi! I have already answer the first question above.
No, before doing our experiment we did not have any idea how our gene would react to DNA damage.
Hydroxyurea is a very strong chemical that induces double stranded breaks in DNA.
curesymposium.org 
Hi, what does it mean for a gene to be up regulated, down regulated, or have no change?
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So when its upregulated, it means the gene is being transcribed more as the cell needs more of the resulting protein in the cell.
Conversely, if it is downregulated, then less of the resulting protein is needed in the cell so smaller amounts of the gene is transcribed.
In this experiment, we are inducing DNA damage so if our gene is involved in DNA repair, the cell would need more of the protein our gene codes for and thus it will be upregulated.
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Hello, can you further explain what it means to be up regulated, down regulated, or for it to stay the same.
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Can the identification of the RDN2 gene as necessary to repair DNA damage help with other health issues besides cancer, or can it help with industries outside of the healthcare field?
LikeLike
It could have to potential to play a role in any biological process/treatment that involves the repair and maintenance of DNA. This might include anti-aging treatments or GMO research.
LikeLike
Hi! Your presentation was great! Is there a way to change the quantity of the RDN2 gene if it presents in small quantities when doing research? Kind of like phages how you are able to genetically engineer them to become lytic after originally being temperate?
LikeLike
You might be able to use a higher cell count in your initial sample however this may have negative effects on your controls. Ideally we would just use a more sensitive technique such as rt-qPCR which would provide a much more quantitative result.
LikeLike
What are the specific methods used that are more sensitive to detecting whether a gene is up regulated? What is cDNA?
LikeLike
Other methods include quantitative PCR which would allow us to quantitatively compare the regulation of the gene before and after DNA damage has been induced.
cDNA (copy DNA) is created using mRNA. Single stranded mRNA is reverse transcribed into double stranded DNA creating a strand of DNA that does not contain any introns.
LikeLike
In the Future Directions portion of your poster you mention that there are alternative approaches to obtaining data about the RDN2 gene. Would you mind elaborating on what the procedure or general basis of these alternative approaches are when compared to your procedure?
LikeLike
Hi! Your presentation as really good! Are there other experiments that you would need to do to confirm your results.
Prior to you experiment, do you know if your specific gene is upregulated, down regulated, etc.
Finally, can you explain the role of hydroxyurea.
Thank you so much 🙂
LikeLike
Hi! I have already answer the first question above.
No, before doing our experiment we did not have any idea how our gene would react to DNA damage.
Hydroxyurea is a very strong chemical that induces double stranded breaks in DNA.
LikeLike