Yeah! We did see very faint gDNA bands when we were expecting brighter ones, and this was likely due to degradation or set-up error since we see it in our control lane as well. We didn’t see cDNA bands because the primer that we designed was on the edge of an intron and an exon, and cDNA doesn’t contain any introns, so this likely caused the lack of cDNA expression.
I don’t think so! We would likely keep the hypothesis the same because we do think that RSP1 is involved in DNA damage repair, and if it is, then we would definitely see that increase in expression!
We used Tetrahymena because they are eukaryotes, so they share some DNA with humans, they are fairly cheap and cost-effective, and they intrinsically have a lot of DNA damage, and therefore have a lot of DNA damage repair, so they are good to use for an experiment like this that looks at DNA damage repair!
I would love to continue on with future directions if I had the chance! I think that this research is super interesting and it can have some very beneficial implications!
I think that it is likely due to the ways that DNA evolved to repair that DNA damage. If DNA and cells evolved to use RSP1 in the DNA damage repair pathway, then likely, yes!
curesymposium.org 
Hi! Could you explain why there are no visible bands for cDNA and gDNA and what went wrong with the primer design to cause this?
LikeLike
Yeah! We did see very faint gDNA bands when we were expecting brighter ones, and this was likely due to degradation or set-up error since we see it in our control lane as well. We didn’t see cDNA bands because the primer that we designed was on the edge of an intron and an exon, and cDNA doesn’t contain any introns, so this likely caused the lack of cDNA expression.
LikeLike
Sounds great! Are there any changes you would make to your hypothesis if you were to complete this project again?
LikeLike
I don’t think so! We would likely keep the hypothesis the same because we do think that RSP1 is involved in DNA damage repair, and if it is, then we would definitely see that increase in expression!
LikeLike
Great video! Why did you specifically study T. thermophila?
LikeLike
We used Tetrahymena because they are eukaryotes, so they share some DNA with humans, they are fairly cheap and cost-effective, and they intrinsically have a lot of DNA damage, and therefore have a lot of DNA damage repair, so they are good to use for an experiment like this that looks at DNA damage repair!
LikeLike
Would you continue on with the future directions if given the chance? Why or why not?
LikeLike
I would love to continue on with future directions if I had the chance! I think that this research is super interesting and it can have some very beneficial implications!
LikeLike
Do you think the induced gene expression due to an evolutionary trait of DNA?
LikeLike
I think that it is likely due to the ways that DNA evolved to repair that DNA damage. If DNA and cells evolved to use RSP1 in the DNA damage repair pathway, then likely, yes!
LikeLike