I think it is likely that we made a loading error when loading this sample into the gel. Since all of our other samples looked good, this is the most likely reason.
I love the layout of your poster, it’s really pleasing to look at and I love how large the figures are! I was wondering if you could elaborate on how you suspect the positive control resulted in a negative result and how you may be able to adjust your methods or possibly materials in an attempt to eliminate that possibility. Would you use another method to test for DNA damage, or repeat the same procedure?
This was likely a loading error when running the gel electrophoresis. We could use our PCR samples and re-do the gel to see if we get an expected band. If there was an issue with PCR we would need to go back to RNA extraction and cDNA synthesis to ensure we have DNA that is able to be shown on the gels.
curesymposium.org 
Are there any specific events that occurred during the experiment that you believe might have caused the expected results to not show up in the PCR?
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I think it is likely that we made a loading error when loading this sample into the gel. Since all of our other samples looked good, this is the most likely reason.
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What would the significance of been if the Twi8 gene had shown and increase after DNA damage?
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This would have been the expected result and would confirm that sRNA’s are part of the DNA damage repair mechanism with DNA damage.
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I love the layout of your poster, it’s really pleasing to look at and I love how large the figures are! I was wondering if you could elaborate on how you suspect the positive control resulted in a negative result and how you may be able to adjust your methods or possibly materials in an attempt to eliminate that possibility. Would you use another method to test for DNA damage, or repeat the same procedure?
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This was likely a loading error when running the gel electrophoresis. We could use our PCR samples and re-do the gel to see if we get an expected band. If there was an issue with PCR we would need to go back to RNA extraction and cDNA synthesis to ensure we have DNA that is able to be shown on the gels.
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What could the medical benefits be for the data?
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In this stage a research there aren’t any specific medical benefits. This is just understanding the role of DNA damage repairs mechanisms.
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What makes this Easy to culture? What other applications would you use for this?
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