That is my mistake, I misspoke in the presentation. Our negative controls are the wells containing -RT. So in all our primers, we have two negative controls: -RT HU and -RT UT. Because no bands were produced in the negative controls as we expected, we can be sure that our results are reliable.
Since our results were inconclusive, I would like to perform the same experiments to determine whether our gene actually has a role in the DNA repair pathway.
If there is no positive control how can you be sure that yours works if you have nothing to compare it to?
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That is my mistake, I misspoke in the presentation. Our negative controls are the wells containing -RT. So in all our primers, we have two negative controls: -RT HU and -RT UT. Because no bands were produced in the negative controls as we expected, we can be sure that our results are reliable.
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What kind of experiment would you do next in your future directions?
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Since our results were inconclusive, I would like to perform the same experiments to determine whether our gene actually has a role in the DNA repair pathway.
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what is the difference between cDNA and gDNA?
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The difference between cDNA and gDNA is gDNA has exons and introns while cDNA does not.
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Do you have any genes you would like to research that you hypothesize have to deal with DNA damage?
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Our gene is homologous to homo sapiens, so it would be interesting to see if the gene in humans would play a role in the DNA damage repair as well.
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