Hello! Your poster mentioned hoping that your research could clarify the processes underpinning genomic stability and integrity. Would you mind describing the degree to which the gene you studied likely contributes to genomic integrity?
Hey Annie, of course!
We believe that RDN2 is very directly involved in maintaining genomic stability and integrity due to the fact that RDN2 gene is found in the nucleolus organizer region (NOR). The replication of the rDNA genes during the cell cycle is controlled by this area. In order to maintain the proper number of rDNA copies and prevent genomic instability, rDNA replication must be properly controlled. This is the tole of RDN2.
In the introduction portion of the poster you mention Tetrahymena Thermophilia being a model candidate for looking at how RDN2 expression is altered. I was wondering if you knew any of the background as to why Tetrahymena Thermophilia has been researched so extensively and if there are any other single celled eukaryotes that could potentially be good candidates for this research?
Hey Kolby, thank you for your question.
Tetrahymena Thermophilia have a genome that has been so extensively studied because it has a relatively large size compared to other unicellular eukaryotes, which makes it a useful model for studying gene expression, functions and regulation. They also have multiple copies of several genes, which allows researchers to study gene expression and regulation in a more complex context. However, I believe that the most attractive fact to use T.Thermophilia as a model candidate is the fact that they can be easily cultured and maintained in the laboratory.
Another option could have been Candida albicans, which is a yeast that is commonly found in the human microbiome. It is known to have been used in DNA repair mechanism research.
Hello! You mentioned that you followed the methodology presented by the Zymo quick RNA miniprep kit, but why did you use this experimental design? Were there any issues you encountered during the investigation?
Hey Channie, thank you very much for your question.
We used the Zymo quick RNA miniprep kit because it is renowned for producing high-quality, very pure RNA, which makes it perfect for use in qPCR, RT-PCR, and RNA sequencing. This purity also guarantees that the isolated RNA is free of DNA contaminants that might obstruct research into DNA repair mechanisms future steps.
Hello. When you mention in your future directions that the goal is to use a more targeted approach in what specific way would the approach be more targeted than the one used for this experiment?
curesymposium.org 
Hello! Your poster mentioned hoping that your research could clarify the processes underpinning genomic stability and integrity. Would you mind describing the degree to which the gene you studied likely contributes to genomic integrity?
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Hey Annie, of course!
We believe that RDN2 is very directly involved in maintaining genomic stability and integrity due to the fact that RDN2 gene is found in the nucleolus organizer region (NOR). The replication of the rDNA genes during the cell cycle is controlled by this area. In order to maintain the proper number of rDNA copies and prevent genomic instability, rDNA replication must be properly controlled. This is the tole of RDN2.
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In the introduction portion of the poster you mention Tetrahymena Thermophilia being a model candidate for looking at how RDN2 expression is altered. I was wondering if you knew any of the background as to why Tetrahymena Thermophilia has been researched so extensively and if there are any other single celled eukaryotes that could potentially be good candidates for this research?
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Hey Kolby, thank you for your question.
Tetrahymena Thermophilia have a genome that has been so extensively studied because it has a relatively large size compared to other unicellular eukaryotes, which makes it a useful model for studying gene expression, functions and regulation. They also have multiple copies of several genes, which allows researchers to study gene expression and regulation in a more complex context. However, I believe that the most attractive fact to use T.Thermophilia as a model candidate is the fact that they can be easily cultured and maintained in the laboratory.
Another option could have been Candida albicans, which is a yeast that is commonly found in the human microbiome. It is known to have been used in DNA repair mechanism research.
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Hello! You mentioned that you followed the methodology presented by the Zymo quick RNA miniprep kit, but why did you use this experimental design? Were there any issues you encountered during the investigation?
LikeLike
Hey Channie, thank you very much for your question.
We used the Zymo quick RNA miniprep kit because it is renowned for producing high-quality, very pure RNA, which makes it perfect for use in qPCR, RT-PCR, and RNA sequencing. This purity also guarantees that the isolated RNA is free of DNA contaminants that might obstruct research into DNA repair mechanisms future steps.
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We did not run into any unexpected issues as we believe to have been very methodical when following guidelines.
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Hello. When you mention in your future directions that the goal is to use a more targeted approach in what specific way would the approach be more targeted than the one used for this experiment?
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