The results of increased expression, visualized through gel electrophoresis, has been seen before with the gene for Rad51, hence why we believe it could very well be involved in the DNA damage repair response pathway.
I could have used any gene known to show increased expression after DNA damage for the positive control, and any gene not involved in the pathway that doesn’t show increased expression for the negative control, maybe like a gene involved in breathing which shouldn’t be effected by dna damage, since we always breathe despite DNA damage and repair constantly happening in our cells.
Template rna was given to us. Reverse transcriptase turned the mRNA to cDNA, which was then cut up by the primers and measured through gel electrophoresis.
There are quite a few genes that show similar patterns of increased expression after DNA damage that could be involved in the pathway, we just only had the time to look at one in this lab.
curesymposium.org 
How do your results here compare to data that has been previously published in the literature?
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The results of increased expression, visualized through gel electrophoresis, has been seen before with the gene for Rad51, hence why we believe it could very well be involved in the DNA damage repair response pathway.
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What other control could you have used?
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I could have used any gene known to show increased expression after DNA damage for the positive control, and any gene not involved in the pathway that doesn’t show increased expression for the negative control, maybe like a gene involved in breathing which shouldn’t be effected by dna damage, since we always breathe despite DNA damage and repair constantly happening in our cells.
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Hello! Great presentation! I was curious how you would test if it is involved in the DNA damage repair pathway.
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I would prevent transcription of the gene and see if DNA damage repair can still occur or if it occurs differently like at a decreased rate.
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How was the mRNA extracted and turned to cDNA?
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Template rna was given to us. Reverse transcriptase turned the mRNA to cDNA, which was then cut up by the primers and measured through gel electrophoresis.
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Hi, I was wondering of there are any other genes that might have a similar effect on the Damage Response Pathway?
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There are quite a few genes that show similar patterns of increased expression after DNA damage that could be involved in the pathway, we just only had the time to look at one in this lab.
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