Your presentation was very well put together! I really enjoyed listening to it. One question I had- why did you use cDNA whenever you were analyzing the RNA? Does this have to do with the instability of RNA, or were there other factors that made you choose to convert to cDNA?
Hi Brayden! I believe we should first extensively research which part of the pathway and to what extent the gene is involved! We could then develop therapies that play to the strengths of Hypedc3 to improve the damage response pathway
I would’ve liked to redo the primer validation experiment just so we can see the annealing of the TWP primers to gDNA. Because we didn’t have a band there, we couldn’t analyze the RPP0 control.
Would you expand on what a CRISPR knockout experiment entails and how that would further your research? As someone with little background knowledge for the research, I am still a bit confused about the CRISPR knockout experiment. Thank you!
Hi Heather! A CRISPR knockout essentially just targets a specific gene sequence using an RNA template that guides the Cas9 protein that’s part of the CRISPR complex. This would allow CRISPR to take out that specific gene/sequence of DNA and join the two ends. We can then see how the cell would react in response to a missing part of its genome. I hope that helps!
curesymposium.org 
Your presentation was very well put together! I really enjoyed listening to it. One question I had- why did you use cDNA whenever you were analyzing the RNA? Does this have to do with the instability of RNA, or were there other factors that made you choose to convert to cDNA?
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Hi Abbey, thank you so much! You’re right, we used cDNA because RNA is unstable and easily degradable
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What were some of the potential therapies that could be developed in light of your research?
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What are some of the potential therapies that could be developed using the knowledge of the Hypedc3 gene?
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Hi Brayden! I believe we should first extensively research which part of the pathway and to what extent the gene is involved! We could then develop therapies that play to the strengths of Hypedc3 to improve the damage response pathway
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What areas would you want to make improvements in your experiment?
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I would’ve liked to redo the primer validation experiment just so we can see the annealing of the TWP primers to gDNA. Because we didn’t have a band there, we couldn’t analyze the RPP0 control.
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Would you expand on what a CRISPR knockout experiment entails and how that would further your research? As someone with little background knowledge for the research, I am still a bit confused about the CRISPR knockout experiment. Thank you!
LikeLike
Hi Heather! A CRISPR knockout essentially just targets a specific gene sequence using an RNA template that guides the Cas9 protein that’s part of the CRISPR complex. This would allow CRISPR to take out that specific gene/sequence of DNA and join the two ends. We can then see how the cell would react in response to a missing part of its genome. I hope that helps!
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