Hi! We were actually assigned this gene and the name, “Hype-C,” was chosen at the beginning of the semester. However, it was originally chosen because its gene expression profile (when mRNA levels are heightened) is similar to that of genes that were already known to be involved in DNA damage repair (which was what we were investigating).
Hello! You were talking about the data being inconclusive and I was wondering what would cause the issue with inconsistent loading and clarity with the bands? Also what can be done to prevent this issue in future testing?
Hi! I believe the data was inconclusive because there was a miscommunication regarding which PCR mixtures (either +RT or -RT) were being loaded into each well. This caused difficulties when comparing the data (because the bands weren’t right next to each other). Now, the clarity issues may be due to inconsistencies in the amount/volume of PCR mixture loaded. To prevent this, labels should be made more clear when loading and PCR mixtures should be mixed more carefully.
curesymposium.org 
Hello! How did you decide to focus on the Hype-C Gene rather than another gene?
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Hi! We were actually assigned this gene and the name, “Hype-C,” was chosen at the beginning of the semester. However, it was originally chosen because its gene expression profile (when mRNA levels are heightened) is similar to that of genes that were already known to be involved in DNA damage repair (which was what we were investigating).
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Hello! You were talking about the data being inconclusive and I was wondering what would cause the issue with inconsistent loading and clarity with the bands? Also what can be done to prevent this issue in future testing?
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Hi! I believe the data was inconclusive because there was a miscommunication regarding which PCR mixtures (either +RT or -RT) were being loaded into each well. This caused difficulties when comparing the data (because the bands weren’t right next to each other). Now, the clarity issues may be due to inconsistencies in the amount/volume of PCR mixture loaded. To prevent this, labels should be made more clear when loading and PCR mixtures should be mixed more carefully.
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