Hi Kieran, as someone who took this lab during the height of COVID, we were not able to do a lot of thew experiments for the lab. So, did you and your group do the reverse transcription of your mRNA?
Hi Cameron, yes we did! We did a reverse transcriptase PCR for the gene specific, rad51, and loading control samples, the results of which are in figure 2 of the poster!
Hey great job! Do you think that there would be a significant loss of protein content in the cell after undergoing the DNA damage from this experiment?
Should the rerun yield the same results, we would like to perform a western blot to assess the actual protein content in the treated cells as well as a gene knockout to see if DNA damage accumulates as we expect it to without RSP-1.
curesymposium.org 
Hi Kieran, as someone who took this lab during the height of COVID, we were not able to do a lot of thew experiments for the lab. So, did you and your group do the reverse transcription of your mRNA?
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Hi Cameron, yes we did! We did a reverse transcriptase PCR for the gene specific, rad51, and loading control samples, the results of which are in figure 2 of the poster!
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Hey great job! Do you think that there would be a significant loss of protein content in the cell after undergoing the DNA damage from this experiment?
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Based on the increased gene expression following DNA damage, we would expect an increase in RSP-1 protein content
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In terms of next steps, what would you do after you complete the re-run and assessing the RSP1’s?
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Should the rerun yield the same results, we would like to perform a western blot to assess the actual protein content in the treated cells as well as a gene knockout to see if DNA damage accumulates as we expect it to without RSP-1.
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Hey Kieran, good poster! How do you predict the DNA damage accumulation will compare in the gene knockout after removing the RSP-1 gene?
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