As stated in the presentation and written on the poster the controls were: the Rpp0 gene (which was the housekeeping control), the Rad51 gene (which was the control to make sure DNA damage was induced, and the +/-RT controls (which controlled for contamination). I go into more depth in my presentation as well!
Great question! I touch on it a little in my presentation, but to go more in depth, the +RT control is when the RNA is reverse transcribed and -RT stands for no reverse transcription. This allows us to see if there is any contamination because no cDNA bands should appear for the -RT sample. If we did see a band for the -RT sample this could be attributed to gDNA contamination. In quantitative data analysis we accounted for this contamination to more accurately reflect levels of cDNA present in the +RT sample.
Great question! We put the Rfc1 cDNA coding sequence into a platform called benchling and then we designed our primers to anneal to this target sequence. After designing primers we attached them to the genome within the benchling software and it autogenerated how many base pairs were included from the start of our forward primer to the end of our reverse primer.
curesymposium.org 
What were the controls used?
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As stated in the presentation and written on the poster the controls were: the Rpp0 gene (which was the housekeeping control), the Rad51 gene (which was the control to make sure DNA damage was induced, and the +/-RT controls (which controlled for contamination). I go into more depth in my presentation as well!
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What does “RT Control” mean? What step/component is it missing that allows us to see contamination?
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Great question! I touch on it a little in my presentation, but to go more in depth, the +RT control is when the RNA is reverse transcribed and -RT stands for no reverse transcription. This allows us to see if there is any contamination because no cDNA bands should appear for the -RT sample. If we did see a band for the -RT sample this could be attributed to gDNA contamination. In quantitative data analysis we accounted for this contamination to more accurately reflect levels of cDNA present in the +RT sample.
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Amazing presentation. How did you determine that your expected base pair length was 520 bp?
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Great question! We put the Rfc1 cDNA coding sequence into a platform called benchling and then we designed our primers to anneal to this target sequence. After designing primers we attached them to the genome within the benchling software and it autogenerated how many base pairs were included from the start of our forward primer to the end of our reverse primer.
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How could we replicate this experiment with human cells to see if Rfc1 also has the same amount of importance
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