BLAST actually was not involved with manipulation of rrm2 in relation to our experiments. This program was used to compare our possible primers to other T. therm genes to ensure selective binding to rrm2. BLAST told us that our primers were not complementary enough to any other sequences to anneal and amplify them.
While we would have to conduct further experiments to be sure, one explanation for the observed primer failure could be degradation or evaporation of the primers themselves at some stage in the preparation of the PCR.
Does the higher concentration of RRM2 in cancer patients indicate that the body is trying to fight the cancer or is it a side product of rapid cell growth and reproduction?
While I would have to do more investigation into this field, I would assume that the increased expression of rrm2 in cancer cells is due to cell proliferation and rapid growth. rrm2 codes for a protein that functions as a ribonuclease reductase, which plays a part in the assembly of DNA. DNA replication (assembly of identical DNA) is a crucial step in division. The higher presence of rrm2 could correlate to faster DNA replication in cancer cells, leading to greater rates of division.
curesymposium.org 
How did the blast work to insert the Rm2?
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BLAST actually was not involved with manipulation of rrm2 in relation to our experiments. This program was used to compare our possible primers to other T. therm genes to ensure selective binding to rrm2. BLAST told us that our primers were not complementary enough to any other sequences to anneal and amplify them.
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Good Job!
Why do you think the primers did not anneal when they were validated?
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While we would have to conduct further experiments to be sure, one explanation for the observed primer failure could be degradation or evaporation of the primers themselves at some stage in the preparation of the PCR.
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Does the higher concentration of RRM2 in cancer patients indicate that the body is trying to fight the cancer or is it a side product of rapid cell growth and reproduction?
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While I would have to do more investigation into this field, I would assume that the increased expression of rrm2 in cancer cells is due to cell proliferation and rapid growth. rrm2 codes for a protein that functions as a ribonuclease reductase, which plays a part in the assembly of DNA. DNA replication (assembly of identical DNA) is a crucial step in division. The higher presence of rrm2 could correlate to faster DNA replication in cancer cells, leading to greater rates of division.
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