I believe that it was just a loading error because as you can see in the image of our gel, there is absolutely no bands in either row for our Rad51 gene. If there was contamination we would likely see bands, but likely not in the expected spots in the gel.
I believe that it was a loading error only because if you look at the gel image, there are absolutely no bands where our Rad51 gene was supposed to appear. If there were gDNA contamination we would likely see something in the Rad51 rows, but probably not at the expected lengths.
DNA damaged was induced by adding hydroxyurea to our cDNA. I believe I stated this in the presentation, but hydroxyurea is a DNA damage inducing agent which is what we treated all of our treated samples with.
Had my partners and I had time to go back and redo our gel, I would try to prevent any gel loading errors so we could have seen bands at the expected lengths in the gel for our Rad51 gene. This way we would have been able to have something to compare the bands of our Dicer-2 gene to and we would have been able to draw a more firm conclusion about the involvement of the Dicer-2 gene in the DNA damage repair process.
I’m not exactly sure what you mean by what mechanism, but as I said in the presentation, if there is an indication that there was an increase in expression of the gene Dicer-2 that means that it has to potential to be involved in the DNA damage repair pathway. Since our Rad51 gene bands did not show up, we cannot confirm that our bands for the Dicer-2 gene definitely mean that our gene is involved in DNA damage repair, but we can state that it is possible because the results were as expected for the Dicer-2 gene if the Rad51 gene had shown up properly.
curesymposium.org 
I know that you said that it was a loading error but was it also a contamination error ?
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I believe that it was just a loading error because as you can see in the image of our gel, there is absolutely no bands in either row for our Rad51 gene. If there was contamination we would likely see bands, but likely not in the expected spots in the gel.
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I believe that it was a loading error only because if you look at the gel image, there are absolutely no bands where our Rad51 gene was supposed to appear. If there were gDNA contamination we would likely see something in the Rad51 rows, but probably not at the expected lengths.
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I know you said it was loading error but could it have been a contamination error that occured?
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How was DNA damage induced?
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DNA damaged was induced by adding hydroxyurea to our cDNA. I believe I stated this in the presentation, but hydroxyurea is a DNA damage inducing agent which is what we treated all of our treated samples with.
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Was there a specific procedure that damaged DNA in this lab?
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Yes, as I stated in my presentation we induced the DNA damage on our treated samples using hydroxyurea which is a DNA inducing agent.
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Is there anything in the experimental process that you would have changed?
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Had my partners and I had time to go back and redo our gel, I would try to prevent any gel loading errors so we could have seen bands at the expected lengths in the gel for our Rad51 gene. This way we would have been able to have something to compare the bands of our Dicer-2 gene to and we would have been able to draw a more firm conclusion about the involvement of the Dicer-2 gene in the DNA damage repair process.
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What type of mechanism are you looking for in the DNA damage repair using Dicer2?
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I’m not exactly sure what you mean by what mechanism, but as I said in the presentation, if there is an indication that there was an increase in expression of the gene Dicer-2 that means that it has to potential to be involved in the DNA damage repair pathway. Since our Rad51 gene bands did not show up, we cannot confirm that our bands for the Dicer-2 gene definitely mean that our gene is involved in DNA damage repair, but we can state that it is possible because the results were as expected for the Dicer-2 gene if the Rad51 gene had shown up properly.
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