Good job presenting you seem like you have great knowledge of your subject matter and were just a bit pressed for time. Could you explain how the process of designing a primer works briefly and for instance why you designed one to fit at that specific position and certain length?
For this lab our primers were designed using online tools. There are various important metrics towards making a proper primer such as the length of the section encompassed by the primers and the CG content of the product. One of the main goals is to make sure that the primers don’t form any secondary structures when they interact with the gene. Since the primers I ended up using did not form these structures, they were determined to be good for usage. The actual position didn’t really matter since the entire gene contains no introns.
Sorry if it was poorly explained. There weren’t two different versions of Rad51. Rather some of my sample contained reverse transcriptase while others did not. This was to make sure there was a lack of contamination as if the sample without reverse transcriptase showed any results something went wrong with the PCR. They effectively serve as a negative control.
Your presentaiton was very informative. For the future direction of testing the human gene, would you be undergoing similar methods? What aspects would differ the most?
I would likely want to keep the testing procedure more or less the same as the goal would be to see if it expresses itself in a manner similar to the Twi7 gene I already tested. The only way it may differ is if the product size of the human gene is different thus forcing me to use a different % gel to visualize it properly. Otherwise, I cannot foresee any need to change the existing procedure.
Your presentation was very informative. For your future directions of testing the human gene, would you undergo similar methods? What would differ the most?
I just commented once and now I can’t tell if this is posting or not. Could you briefly explain the purpose and process of the primer design? For instance how you know which location it should bind to and the length to try and make it?
Very informative presentation. For your future direciton about testing the human gene, would you go through similar methods? What would be the most difference?
I know that other groups in my lab performed similar tests to my own on other Twi genes in the tetrahymena cells. I’d like to test those myself to see if they behave similarly as a result of their similar make up and positions in the genetic code.
curesymposium.org 
Good job presenting you seem like you have great knowledge of your subject matter and were just a bit pressed for time. Could you explain how the process of designing a primer works briefly and for instance why you designed one to fit at that specific position and certain length?
LikeLike
For this lab our primers were designed using online tools. There are various important metrics towards making a proper primer such as the length of the section encompassed by the primers and the CG content of the product. One of the main goals is to make sure that the primers don’t form any secondary structures when they interact with the gene. Since the primers I ended up using did not form these structures, they were determined to be good for usage. The actual position didn’t really matter since the entire gene contains no introns.
LikeLike
Very detailed presentation. What were the two versions of rad51 and what is the significance of using two versions of rad51 in your experiment?
LikeLike
Sorry if it was poorly explained. There weren’t two different versions of Rad51. Rather some of my sample contained reverse transcriptase while others did not. This was to make sure there was a lack of contamination as if the sample without reverse transcriptase showed any results something went wrong with the PCR. They effectively serve as a negative control.
LikeLike
Your presentaiton was very informative. For the future direction of testing the human gene, would you be undergoing similar methods? What aspects would differ the most?
LikeLike
I apologize this comment posted multiple times, it was only intended to be once.
LikeLike
I would likely want to keep the testing procedure more or less the same as the goal would be to see if it expresses itself in a manner similar to the Twi7 gene I already tested. The only way it may differ is if the product size of the human gene is different thus forcing me to use a different % gel to visualize it properly. Otherwise, I cannot foresee any need to change the existing procedure.
LikeLike
Your presentation was very informative. For your future directions of testing the human gene, would you undergo similar methods? What would differ the most?
LikeLike
I just commented once and now I can’t tell if this is posting or not. Could you briefly explain the purpose and process of the primer design? For instance how you know which location it should bind to and the length to try and make it?
LikeLike
Very informative presentation. For your future direciton about testing the human gene, would you go through similar methods? What would be the most difference?
LikeLike
I think you did a great job do you have any idea what similar gene as Twi7 in tetrahymena thermophila you’d look for?
LikeLike
I know that other groups in my lab performed similar tests to my own on other Twi genes in the tetrahymena cells. I’d like to test those myself to see if they behave similarly as a result of their similar make up and positions in the genetic code.
LikeLike
Very nice job at the presentation!! I was wondering though, what other kind of genes are similar to Twi7 in tetrahymena thermophila?
LikeLike
If damaging the DNA does not affect Twi7 expression, what could potentially affect Twi7 expression and would this be worthwhile to study?
LikeLike