If you retested this entire experiment and did not have a contaminated positive control, what is your prediction of the results? Would it mirror the assumption you made that Dicer-2 is a part of DNA damage repair?
Great Job! You mentioned that moving forward you would like to find a “better primer.” Can you explain what you mean by better or what characteristics are you looking for to find another primer?
Do you have an idea how your positive control got contaminated?
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What primer would you use in the future ?
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If you retested this entire experiment and did not have a contaminated positive control, what is your prediction of the results? Would it mirror the assumption you made that Dicer-2 is a part of DNA damage repair?
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What new primer would you use in the future?
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Can you compare the rate at which siRNA Dicer2 repairs DNA compared to other siRNAs? How would you do this?
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Great Job! You mentioned that moving forward you would like to find a “better primer.” Can you explain what you mean by better or what characteristics are you looking for to find another primer?
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