I think that I would continue to use T. thermophila for both studying Hypedc3 and for other potential genes because: the Hypedc3 we studied was from this organism so it would be good to study its other potential gene pathway involvements in this organism; and T. thermophila has been a great model organism for researching DNA damage repair for numerous studies throughout the years.
The main characteristics of the gene were that during conjugation, it showed expression level peaks at C2, C4, and C6 and dips at similar times as well to genes that we know are involved in DNA damage repair like RAD51.
I would say that we would look for similar characteristics as we did for Hypedc3: examining the conjugation patterns and comparing them to those of RAD51 and HOP3 since we know those two are involved in DNA damage repair.
Thank you! The gDNA bands did not appear on our gel because it most likely got degraded; this is especially supported by the fact that the gDNA bands did not appear for our entire lab sections.
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would you continue to test using the same model organism, or switch to a different one?
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I think that I would continue to use T. thermophila for both studying Hypedc3 and for other potential genes because: the Hypedc3 we studied was from this organism so it would be good to study its other potential gene pathway involvements in this organism; and T. thermophila has been a great model organism for researching DNA damage repair for numerous studies throughout the years.
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What was the main characteristic about the gene that drew you to study it in this lab?
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The main characteristics of the gene were that during conjugation, it showed expression level peaks at C2, C4, and C6 and dips at similar times as well to genes that we know are involved in DNA damage repair like RAD51.
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You said it is important to look for other potential genes for DNA damage repair, what characteristics would other potential genes have?
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I would say that we would look for similar characteristics as we did for Hypedc3: examining the conjugation patterns and comparing them to those of RAD51 and HOP3 since we know those two are involved in DNA damage repair.
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You did such a good job! Do you have a hypothesis as to why the two bands did not show in your gel?
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Thank you! The gDNA bands did not appear on our gel because it most likely got degraded; this is especially supported by the fact that the gDNA bands did not appear for our entire lab sections.
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