Comparison between the twi2 gene and cancer genes could be done through similar processes described in this presentation. Understanding the DNA damage repair pathway in general is beneficial for cancer research.
During the experimentation portion, were there any parts that were particularly difficult? Or a method that you had to repeat multiple times to receive proper results?
One of the experiments that was particularly tedious was the RNA extraction experiment. RNases are everywhere, so we had to be very aware of our surroundings and careful to avoid contamination. Luckily, all of our experiments produced positive results so we did not have to repeat any.
curesymposium.org 
Great presentation. How would you knock out the Twi2 gene in your future experiments?
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I would perform a gene knockout using CRISPR-Cas9
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Could research be done to investigate the correlation between Twi2 mutations and cancer? if so how would this be done?
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Comparison between the twi2 gene and cancer genes could be done through similar processes described in this presentation. Understanding the DNA damage repair pathway in general is beneficial for cancer research.
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During the experimentation portion, were there any parts that were particularly difficult? Or a method that you had to repeat multiple times to receive proper results?
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One of the experiments that was particularly tedious was the RNA extraction experiment. RNases are everywhere, so we had to be very aware of our surroundings and careful to avoid contamination. Luckily, all of our experiments produced positive results so we did not have to repeat any.
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You chose your subject based on its high GC content why exactly?
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