Hi Hawk, the primer is designed with the intention of it annealing to a certain sequence of the genome, and this means that there may have been another repeat of this exact sequence at another location of the genome, and if the primer annealed here accidentally, it can result in an incorrect length. Additionally, since all of our bands consistently had the same amount of error, it could be due to too much or too little of the ladder being added. This could result in an overall incorrect reading.
A possible reason for this is could be that there ma
The primer is designed with the intention of it annealing to a certain sequence of the genome, and this means that there may have been another repeat of this exact sequence at another location of the genome, and if the primer annealed here accidentally, it can result in an incorrect length.
Since RFC1 is designed specifically for a genome, it cannot be altered, however, it could be possible to use a completely different genome or replication factor in order to get it to possibly anneal correctly.
curesymposium.org 
Why do you think that your primers annealed incorrectly?
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Hi Hawk, the primer is designed with the intention of it annealing to a certain sequence of the genome, and this means that there may have been another repeat of this exact sequence at another location of the genome, and if the primer annealed here accidentally, it can result in an incorrect length. Additionally, since all of our bands consistently had the same amount of error, it could be due to too much or too little of the ladder being added. This could result in an overall incorrect reading.
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What is a possible explanation for the primers being active but in the wrong spots?
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A possible reason for this is could be that there ma
The primer is designed with the intention of it annealing to a certain sequence of the genome, and this means that there may have been another repeat of this exact sequence at another location of the genome, and if the primer annealed here accidentally, it can result in an incorrect length.
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How can you correct or alter RFC1 in your second PCR to be annealed correctly?
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Since RFC1 is designed specifically for a genome, it cannot be altered, however, it could be possible to use a completely different genome or replication factor in order to get it to possibly anneal correctly.
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