I do not believe we dealt with any cDNA degradation because of the presence of bands in expected lanes, but if we did have and degradation we likely would have had to run the PCRs again with new cDNA that was not degraded in order to obtain results.
So the lack of a band could have been caused by a number of reasons, but the most likely reason is that we just did not add primers to the PCR that was put in the lane 11 well. You cannot really see a primer dimer at the bottom of lane 11 like you can see in the other lanes, so it is likely that we missed that tube when adding primers to our PCRs.
What is the big picture of this research project? I know you mentioned you were researching RNA pathways but what relevance does that hold in the scientific community?
DNA damage is unavoidable and can cause various negative effects, including cancer, so it’s super important that we know how our cells are able to repair DNA damage and protect us from things like cancer. We still don’t know how exactly these pathways work, so the research I presented was focused on a pretty specific part of the pathway. Although it was specific, it is a part of the bigger picture of learning how cells repair DNA damage that can have so many negative effects on our health so that we can strengthen the cell’s ability to protect us.
I answered a similar question below your, but basically, this research is relevant because DNA damage is unavoidable and, although we know some things about how cells go about repairing it, we don’t know all of the specifics such as what proteins are vital for damage repair (that’s what was researched here).
We expected identical bands of 550 base pairs in both lanes 11 and 12, but only lane 12 displayed the expected result. The PCRs in these lanes were meant to amplify Rpp0, a housekeeping gene that should have equal levels of expression under all conditions (hence the expected results being identical bands in both lanes), so for this experiment, it was supposed to be our loading control. I answered a question above explaining why this may have happened!
curesymposium.org 
Throughout your experiments, did you experience any DNA degradation and how did you handle that?
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I do not believe we dealt with any cDNA degradation because of the presence of bands in expected lanes, but if we did have and degradation we likely would have had to run the PCRs again with new cDNA that was not degraded in order to obtain results.
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Why do you think you only had 1 band instead of 2 in the loading control?
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So the lack of a band could have been caused by a number of reasons, but the most likely reason is that we just did not add primers to the PCR that was put in the lane 11 well. You cannot really see a primer dimer at the bottom of lane 11 like you can see in the other lanes, so it is likely that we missed that tube when adding primers to our PCRs.
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What can your research be applied towards in a general sense? What further research will this be used for?
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What is the big picture of this research project? I know you mentioned you were researching RNA pathways but what relevance does that hold in the scientific community?
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DNA damage is unavoidable and can cause various negative effects, including cancer, so it’s super important that we know how our cells are able to repair DNA damage and protect us from things like cancer. We still don’t know how exactly these pathways work, so the research I presented was focused on a pretty specific part of the pathway. Although it was specific, it is a part of the bigger picture of learning how cells repair DNA damage that can have so many negative effects on our health so that we can strengthen the cell’s ability to protect us.
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I answered a similar question below your, but basically, this research is relevant because DNA damage is unavoidable and, although we know some things about how cells go about repairing it, we don’t know all of the specifics such as what proteins are vital for damage repair (that’s what was researched here).
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What were the unexpected results in lane 11?
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We expected identical bands of 550 base pairs in both lanes 11 and 12, but only lane 12 displayed the expected result. The PCRs in these lanes were meant to amplify Rpp0, a housekeeping gene that should have equal levels of expression under all conditions (hence the expected results being identical bands in both lanes), so for this experiment, it was supposed to be our loading control. I answered a question above explaining why this may have happened!
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What were the undesired results in lane 11?
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