If our gene Rsp-1 is harmed or loses effect due to a disease or cancer, being able to target this gene to alter it, replace it, or help it get back to a functioning state, which requires genetic engineering, could be extremely beneficial and save the cell from dying if it couldn’t repair the DNA damage.
The band brightness correlates with the gene’s expression. When we induce DNA damage, if a band is brighter than an untreated version (UT), then our gene expression increased which increases the band’s brightness. That brightness tells us that the gene has some role in DNA damage repair since its expression (band brightness) increased after we induced DNA damage (+HU).
There were many contingencies when designing our gene specific primers. Our primers could have folded onto themselves, or they could have been degraded during the overall process of adding them to PCRs which would also prevent them from annealing successfully. Overall, we are not quite sure why they didn’t anneal, but we have several theories as to what could have happened.
curesymposium.org 
How could the Rsp1 gene be used for genetic engineering?
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If our gene Rsp-1 is harmed or loses effect due to a disease or cancer, being able to target this gene to alter it, replace it, or help it get back to a functioning state, which requires genetic engineering, could be extremely beneficial and save the cell from dying if it couldn’t repair the DNA damage.
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Great presentation! Why are bands brighter after DNA damage has occurred?
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The band brightness correlates with the gene’s expression. When we induce DNA damage, if a band is brighter than an untreated version (UT), then our gene expression increased which increases the band’s brightness. That brightness tells us that the gene has some role in DNA damage repair since its expression (band brightness) increased after we induced DNA damage (+HU).
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Why do you think that the primers you designed didn’t anneal?
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There were many contingencies when designing our gene specific primers. Our primers could have folded onto themselves, or they could have been degraded during the overall process of adding them to PCRs which would also prevent them from annealing successfully. Overall, we are not quite sure why they didn’t anneal, but we have several theories as to what could have happened.
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