Hey great presentation! Do you think that the DNA damage would have a significant effect on the protein content (the potential quality and quantity of the protein) if you moved forward with what you discussed in your future directions?
Thank you and that’s a great question! I would assume so and that would be a great research question moving forward. It makes sense that DNA damage could cause mutations that are either silent or can result in protein issues.
Which primer would you move forward with, as both seem to be expressed in the first electrophoresis, and you mention that c primer does not bind to the correct sequence but how is this known?
Great question! We would continue with using the same primers due to the gel validation that it does successfully bind to the gDNA. It also was confirmed it binds to the cDNA due to the second gel having a faint band.
curesymposium.org 
Hey great presentation! Do you think that the DNA damage would have a significant effect on the protein content (the potential quality and quantity of the protein) if you moved forward with what you discussed in your future directions?
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Thank you and that’s a great question! I would assume so and that would be a great research question moving forward. It makes sense that DNA damage could cause mutations that are either silent or can result in protein issues.
LikeLike
Which primer would you move forward with, as both seem to be expressed in the first electrophoresis, and you mention that c primer does not bind to the correct sequence but how is this known?
LikeLike
Great question! We would continue with using the same primers due to the gel validation that it does successfully bind to the gDNA. It also was confirmed it binds to the cDNA due to the second gel having a faint band.
LikeLike
Great poster! What biological features makes tetrahymena thermophila have an advantage to experiment on?
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Thank you and great question! Tetrahymena is a ideal model organism due to it being easy to culture, inexpensive, and easy to access.
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Would there be a point in time where the damage to the DNA become to great for repairs to occur?
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