Hey Zeb, successful annealing means that our primers are able to bind to single stranded DNA to initiate elongation, which is what allows us to amplify the DNA and subsequently measure it.
Hey Jenna, we would have liked to see very similar results to the Rad51 lanes in the TWI8 lanes to confirm that it was involved in the DNA repair pathway.
Hey Mayleen, I have a few theories, but the most likely to me is that the wells were messed up in gel we used for electrophoresis, meaning I think they were not deep enough or not fully formed.
Great job, biochem major here and it was really cool seeing an experiment on the processes I’ve been learning about. One question I have is – What do you think led the error in gel electrophoresis in Piwi8 UT and HT? The primer data looked good so I wonder what theories you may have.
Hey Albert, glad you enjoyed! My strongest theory is that the gel we used in the electrophoresis experiment was messed up, seeing as the ladder was bent. There isn’t really any other likely explanations as to why this happened. Likely the wells that we placed our samples in were not deep enough.
Hey Alexander, for this experiment specifically, there would likely not be much of a different response as we weren’t measuring for a specific type of DNA damage. We were more exploring the pathway involved in the repair of the damage, which would come about from any form of DNA damage that wasn’t severe enough to outright kill the cell.
Hey Avery, a definitive result for this experiment would allow us to conclude that our gene is highly likely to be involved in the DNA repair pathway, or that it is likely not involved in the DNA repair pathway.
curesymposium.org 
What does successful annealing to your gene of interest mean(what does that result tell us for the overall experiment)?
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Hey Zeb, successful annealing means that our primers are able to bind to single stranded DNA to initiate elongation, which is what allows us to amplify the DNA and subsequently measure it.
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what would figure 2 look like if the results turned out how you wanted?
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Hey Phoebe, figure two would have shown a similar result to Rad51 in the experimental lanes if TWI8 was involved in the DNA repair pathway.
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What does successful annealing to your gene of interest represent(what does that result mean for the overall experiment)?
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What were the expected results to support your hypothesis?
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Hey Jenna, we would have liked to see very similar results to the Rad51 lanes in the TWI8 lanes to confirm that it was involved in the DNA repair pathway.
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Do you have an idea as to why TW18 went wrong?
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Hey Mayleen, I have a few theories, but the most likely to me is that the wells were messed up in gel we used for electrophoresis, meaning I think they were not deep enough or not fully formed.
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Do you think that the error in Figure 2 was a result of the gel (because the DNA Ladder was messed up) or something else?
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Hey Andy, you hit the nail on the head, I’m fairly certain that was the reason as it is very odd that the ladder was bent.
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Great job, biochem major here and it was really cool seeing an experiment on the processes I’ve been learning about. One question I have is – What do you think led the error in gel electrophoresis in Piwi8 UT and HT? The primer data looked good so I wonder what theories you may have.
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Hey Albert, glad you enjoyed! My strongest theory is that the gel we used in the electrophoresis experiment was messed up, seeing as the ladder was bent. There isn’t really any other likely explanations as to why this happened. Likely the wells that we placed our samples in were not deep enough.
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What would be the effect of using a different DNA damaging agent?
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Hey Alexander, for this experiment specifically, there would likely not be much of a different response as we weren’t measuring for a specific type of DNA damage. We were more exploring the pathway involved in the repair of the damage, which would come about from any form of DNA damage that wasn’t severe enough to outright kill the cell.
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What does a definitive result mean for this experiment
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Hey Avery, a definitive result for this experiment would allow us to conclude that our gene is highly likely to be involved in the DNA repair pathway, or that it is likely not involved in the DNA repair pathway.
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