We hypothesized that expression would increase because expression of Wd40 has been shown to increase during conjugation. Conjugation is a stage when the DNA damage occurs and is repaired. Because the expression increases during conjugation, we hypothesized that Wd40 would be involved in the DNA damage response pathway.
Primer3Plus is a website that allows you to chose from a variety of primers that will likely anneal to your sequence. It also provides stats on the different primers, including GC content and melting point.
curesymposium.org 
Was your group expecting there to be DNA damaged cells or were you surprised in those results?
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Yes, we used hydroxyurea to cause DNA damage in the cells so that we could see if expression of Wd40 increased when DNA damage was induced.
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What made you hypothesize that the expression of the gene would increase?
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We hypothesized that expression would increase because expression of Wd40 has been shown to increase during conjugation. Conjugation is a stage when the DNA damage occurs and is repaired. Because the expression increases during conjugation, we hypothesized that Wd40 would be involved in the DNA damage response pathway.
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What is Primer3 Plus and how was it used to design the primers?
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Primer3Plus is a website that allows you to chose from a variety of primers that will likely anneal to your sequence. It also provides stats on the different primers, including GC content and melting point.
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