Hi Payton! Thank you for your question! 🙂 The reason as to why our RPP0 cDNA band was so highly expressed was because RPP0 was our positive control and it was used to ensure that our designed primers would adequately anneal to cDNA. The appearance of this band was essential to our primer validation and gave us the green light to continue our research using these primers.
Hi Carly! Thank you so much for your question. 🙂 The Hypedc3 gene was chosen to study because we noticed similar patterns between the expression of the Hypedc3 and genes that are known to be involved in DNA Damage Repair which lead us to our Hypothesis that the Hypcedc3 gene was involved in DNA damage response pathways!
curesymposium.org 
Could you explain why the band in the first PCR image is so extremely bright?
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Could you explain why the band in the middle of the first PCR image is so extremely bright and clear?
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Could you explain why the band in the middle of the first PCR gel image is so vibrant and clear? What specific result made it show up in this way?
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Hi Payton! Thank you for your question! 🙂 The reason as to why our RPP0 cDNA band was so highly expressed was because RPP0 was our positive control and it was used to ensure that our designed primers would adequately anneal to cDNA. The appearance of this band was essential to our primer validation and gave us the green light to continue our research using these primers.
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What properties did Hypedc3 have that caused you to choose it?
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Hi Carly! Thank you so much for your question. 🙂 The Hypedc3 gene was chosen to study because we noticed similar patterns between the expression of the Hypedc3 and genes that are known to be involved in DNA Damage Repair which lead us to our Hypothesis that the Hypcedc3 gene was involved in DNA damage response pathways!
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