We would utilize a technique like CRSIPR to knockout gene Wd40, what this would allow us to do would be we can now explore what functions do/don’t work and we would have a better understanding of what exactly Wd40 does during DNA repair.
We were told not to say this is the presentation or on the poster, but during the initial PCR our caps flew open when in the thermocycler and this led to evaporation and whatever wasn’t evaporated was most likely degraded because of the extreme temperature.
curesymposium.org 
what does further exploration of wd40 using gene knockout entail? what new knowledge would these experiments provide?
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We would utilize a technique like CRSIPR to knockout gene Wd40, what this would allow us to do would be we can now explore what functions do/don’t work and we would have a better understanding of what exactly Wd40 does during DNA repair.
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What does it mean for the gene to be ‘upregulated?’
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When a gene is more “upregulated” it means there are more copies of the gene being made and it is being expressed more.
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What does it mean for a gene to be upregulated?
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When a gene is more “upregulated” it means there are more copies of the gene being made and it is being expressed more.
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Why did the control not produce a band? And why were you not able to see the results on gel electrophoresis? What other primer would you use?
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We were told not to say this is the presentation or on the poster, but during the initial PCR our caps flew open when in the thermocycler and this led to evaporation and whatever wasn’t evaporated was most likely degraded because of the extreme temperature.
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