We used DI water as a control as that’s what we dissolved the naloxone (normally a crystalline powder) in. We wanted to make sure that the solvent wasn’t having an antibacterial effect independent of our compound.
We started from a 10 uM level as that is the maximum amount obtainable in human blood, and then we reduced each step by half, down to the .625 uM concentration, as we figured any concentrations past that point would be excessively diluted.
We started at the 10 uM level as that is the maximum concentration achievable in human blood, and then we reduced concentration by half at each step. We stopped at .625 uM because any concentrations past that point would be needlessly diluted.
curesymposium.org 
Do you have a guess as to why the Naloxone gave mixed results?
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I believe that our mixed results were most likely just do to pipetting errors, as titrations from the same stock yielded different results.
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What exactly would you expect when you say, “react more strongly,” when discussing your future directions?
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I’m expecting a more visible antibacterial effect when applied in future experiments as that was what our max-dose experiment was trending towards.
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Great presentation! Why did you use D1 water as a control?
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We used DI water as a control as that’s what we dissolved the naloxone (normally a crystalline powder) in. We wanted to make sure that the solvent wasn’t having an antibacterial effect independent of our compound.
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How did you decide what dilutions to use for the dose response?
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We started from a 10 uM level as that is the maximum amount obtainable in human blood, and then we reduced each step by half, down to the .625 uM concentration, as we figured any concentrations past that point would be excessively diluted.
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We started at the 10 uM level as that is the maximum concentration achievable in human blood, and then we reduced concentration by half at each step. We stopped at .625 uM because any concentrations past that point would be needlessly diluted.
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