To determine if it is an effective antimicrobial, find the range: the mean ± 2 standard deviations of the negative control (50% DMSO). if the absorbances in the well are below 2 standard deviations, then this means that the drug was effective at killing the bacteria in the well, hence there was little bacteria in the well and little absorbance.
There are many techniques you can use. UV-vis spectroscopy and Transmission Electron Microscopy (TEM) are two that are commonly used to characterize particles (i.e measure the size and concentration, test how stable the particles are in solution).
To find the most efficient combination of concentration and size of particles, we could separately run assays to test the effect of concentration of AgNPs on Salmonella Typhimirium and the effect of size of particle on S. Typhimirium respectively. For example, similarly to the way we tested our assays, each the concentration of particle per mL in consecutive well in our plate, decreased/was diluted by 50% from the previous well. Our max dose was at 10 mg/mL and we went all the way down to 0.20 mg/ml. In terms of testing the particle size, UV-vis spectroscopy can be used to assess the SPR (surface plasmon resonance). The maximum wavelength on the SPR curve can be found and used to determine the size of the nanoparticle. I’m assuming spectrophotometry can also be used to assess if the respective size of the nanoparticle effectively kills the bacteria, however the plates may have to be run at a different wavelength depending on the size of the nanoparticle. Creating charts of size vs absorbance and concentration vs absorbance can then be analyzed to find the efficient combination.
In the video it states that Colloidial silver is non-toxic, however doesn’t it also have other adverse side effects such as the staining of skin and affect on other drugs that cause it to be unsafe in humans?
At a much higher dosage than 10 mg/ml, collodial silver definitely induces these effects. However the physiological safe dosage of the drug is 10 mg/ml. So, there wouldnt be any severe effects of the drug.
curesymposium.org 
Good presentation. I was wondering why your graphs show percentages instead of concentrations? Also what were the limitations for your project?
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We wanted to show how much we are diluting in each column from the max dose (10 mg/ml). In this case the max dose equates 100%.
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Really good presentation! How much bacteria in a sample needs to be killed for it to be statistically significant?
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To determine if it is an effective antimicrobial, find the range: the mean ± 2 standard deviations of the negative control (50% DMSO). if the absorbances in the well are below 2 standard deviations, then this means that the drug was effective at killing the bacteria in the well, hence there was little bacteria in the well and little absorbance.
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How could you test for the ideal size and concentration of silver particles to determine the most efficient combination?
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There are many techniques you can use. UV-vis spectroscopy and Transmission Electron Microscopy (TEM) are two that are commonly used to characterize particles (i.e measure the size and concentration, test how stable the particles are in solution).
To find the most efficient combination of concentration and size of particles, we could separately run assays to test the effect of concentration of AgNPs on Salmonella Typhimirium and the effect of size of particle on S. Typhimirium respectively. For example, similarly to the way we tested our assays, each the concentration of particle per mL in consecutive well in our plate, decreased/was diluted by 50% from the previous well. Our max dose was at 10 mg/mL and we went all the way down to 0.20 mg/ml. In terms of testing the particle size, UV-vis spectroscopy can be used to assess the SPR (surface plasmon resonance). The maximum wavelength on the SPR curve can be found and used to determine the size of the nanoparticle. I’m assuming spectrophotometry can also be used to assess if the respective size of the nanoparticle effectively kills the bacteria, however the plates may have to be run at a different wavelength depending on the size of the nanoparticle. Creating charts of size vs absorbance and concentration vs absorbance can then be analyzed to find the efficient combination.
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In the video it states that Colloidial silver is non-toxic, however doesn’t it also have other adverse side effects such as the staining of skin and affect on other drugs that cause it to be unsafe in humans?
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At a much higher dosage than 10 mg/ml, collodial silver definitely induces these effects. However the physiological safe dosage of the drug is 10 mg/ml. So, there wouldnt be any severe effects of the drug.
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