Hi Danya!
Since the rest of the concentrations follow the trend I do not think it was due to an error in creating the dilution series but rather pipetting too much of the 3.13% dilution with possibly a combination of not enough Salmonella. This would definitely skew the results too look more effective than it actual is.
You stated that you found a strange result for the concentration of 3.13% and that it could be attributed to human error. What step do you believe the error was made in, and what was that error?
Hi Lynae, with the research so far conducted we are not 100% sure it is an error. I would like to repeat the procedure again because there is a real possibility that this concentration is the optimal dosage for antibiotic since the data indicates its a strong hit. However, I am more likely to believe it is an error just because there was a very distinct linear trend amongst the other dosages. Further testing is required to make the official conclusion.
You stated that you found a strange result for the concentration of 3.13% due to human error, what step would you attribute the human error to?
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Hi Danya!
Since the rest of the concentrations follow the trend I do not think it was due to an error in creating the dilution series but rather pipetting too much of the 3.13% dilution with possibly a combination of not enough Salmonella. This would definitely skew the results too look more effective than it actual is.
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You stated that you found a strange result for the concentration of 3.13% and that it could be attributed to human error. What step do you believe the error was made in, and what was that error?
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How was the absorption calculated?
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Hi Jade, the absorption is measured using a spectrophotometer at the wavelength 620nm.
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what made you sure that the 3.13 was a error?
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Hi Lynae, with the research so far conducted we are not 100% sure it is an error. I would like to repeat the procedure again because there is a real possibility that this concentration is the optimal dosage for antibiotic since the data indicates its a strong hit. However, I am more likely to believe it is an error just because there was a very distinct linear trend amongst the other dosages. Further testing is required to make the official conclusion.
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