I feel like your video was presented extremely well. As someone who isn’t in this lab it is sometimes hard to read and understand the information on the poster, but I thought you explained it throughly. What future experiment would you look forward the most too?
Hi Madison, thank you so much for your kind feedback! I would like to replicate this experiment and also learn more about potential antibiotic compounds in other types of fungi, as mycology is an interest of mine!
Hi Richy, thanks for your question! Absorbance is measuring the amount of bacteria. If the absorbance is high, that means there is a high amount of bacteria in the wells, meaning the compound did not work as an antibiotic. For an antibiotic to be effective, we would want to see a low absorbance value, because this indicates there is not much bacteria in the well.
Hi Andrew, to be honest I am not 100% sure why that was the case, but we learned this semester that some compounds are just not as effective in high doses and are more effective at lower doses, and vice versa.
Hi Ronald, I would need to do a literature review to properly decide on which compounds I would want to experiment with going forward. However, I think it would be interesting to try testing with compounds from Lion’s Mane mushrooms as I know there has been interesting research done on this type of fungi.
Why do you think that there were no hits in your first figure of results? What changes were made in order to receive a hit that is shown from you second figure in the results?
Hi Asadel, good question! We only found hits in certain, lower concentrations of 1mg/mL and 0.001 mg/mL Betulin (as seen in Figure 2). Figure 1 shows results from high concentrations (none of which were hits).
This suggests that Betulin is a better antibiotic at lower concentrations.
However, it was puzzling that the concentrations in between our hits (0.1 and 0.01 mg/mL) were not also hits. To tease out why this was the case, I would like to replicate this experiment and also test Betulin at lower concentrations to see if there is really a “sweet spot” that corresponds to the concentrations of Betulin that will have the best antibiotic activity.
curesymposium.org 
I feel like your video was presented extremely well. As someone who isn’t in this lab it is sometimes hard to read and understand the information on the poster, but I thought you explained it throughly. What future experiment would you look forward the most too?
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Hi Madison, thank you so much for your kind feedback! I would like to replicate this experiment and also learn more about potential antibiotic compounds in other types of fungi, as mycology is an interest of mine!
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What is absorbance measuring in this scenario and why are absorbance results used to determine the effectiveness of the antibiotic?
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Hi Richy, thanks for your question! Absorbance is measuring the amount of bacteria. If the absorbance is high, that means there is a high amount of bacteria in the wells, meaning the compound did not work as an antibiotic. For an antibiotic to be effective, we would want to see a low absorbance value, because this indicates there is not much bacteria in the well.
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Why do you think lower concentrations worked better as an antibiotic?
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Hi Andrew, to be honest I am not 100% sure why that was the case, but we learned this semester that some compounds are just not as effective in high doses and are more effective at lower doses, and vice versa.
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What other antibiotic compounds would you like to explore in other fungi and how could these differ from Betulin?
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Hi Ronald, I would need to do a literature review to properly decide on which compounds I would want to experiment with going forward. However, I think it would be interesting to try testing with compounds from Lion’s Mane mushrooms as I know there has been interesting research done on this type of fungi.
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Why do you think that there were no hits in your first figure of results? What changes were made in order to receive a hit that is shown from you second figure in the results?
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Hi Asadel, good question! We only found hits in certain, lower concentrations of 1mg/mL and 0.001 mg/mL Betulin (as seen in Figure 2). Figure 1 shows results from high concentrations (none of which were hits).
This suggests that Betulin is a better antibiotic at lower concentrations.
However, it was puzzling that the concentrations in between our hits (0.1 and 0.01 mg/mL) were not also hits. To tease out why this was the case, I would like to replicate this experiment and also test Betulin at lower concentrations to see if there is really a “sweet spot” that corresponds to the concentrations of Betulin that will have the best antibiotic activity.
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