I would start at the max dose. I don’t have huge toxicity concerns with this compound due to it being produced in various mammals and present in various dairy products.
Definitely the inhibition of bacterial growth at the max dose concentration. That experiment was conducted twice with similar results from both experiments. While not as effective as ampicillin which is a current antibiotic, the lactoferrin was either killing or inhibiting further growth. Unfortunately, the bactericidal and bacteriostatic experiments were inconclusive and is something with time I would like to conduct again.
The max dose experiment is the concentration achievable in human blood. The goal of our experiments is to attempt to keep this as physiologically relevant as possible despite working with a well plate. The serial dilution is to find concentrations for where the compound stops working. Higher concentrations can produce potential side effects and toxicity concerns. Lower concentrations of compounds while still producing positive results on bacterial inhibition may be more beneficial to host cells and avoiding adverse side effects
The max dose is relative to concentrations achieved in human blood. If I was to move forward with in-vivo testing in mice I would start with that max dose due to the positive results of bacteria inhibition at that concentration and then evaluate for any toxic effects.
curesymposium.org 
How would you assign a dose if the lactopherrin were to be used in vivo?
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I would start at the max dose. I don’t have huge toxicity concerns with this compound due to it being produced in various mammals and present in various dairy products.
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What would you say the most important result/finding in all of your experiments?
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Definitely the inhibition of bacterial growth at the max dose concentration. That experiment was conducted twice with similar results from both experiments. While not as effective as ampicillin which is a current antibiotic, the lactoferrin was either killing or inhibiting further growth. Unfortunately, the bactericidal and bacteriostatic experiments were inconclusive and is something with time I would like to conduct again.
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Great job! What are the purposes of the max dose and the serial dilutions experiment for you research?
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The max dose experiment is the concentration achievable in human blood. The goal of our experiments is to attempt to keep this as physiologically relevant as possible despite working with a well plate. The serial dilution is to find concentrations for where the compound stops working. Higher concentrations can produce potential side effects and toxicity concerns. Lower concentrations of compounds while still producing positive results on bacterial inhibition may be more beneficial to host cells and avoiding adverse side effects
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Do you believe the max dose should be used in every patient being tested. Including other mammals. Or would the max dose need to be modified?
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The max dose is relative to concentrations achieved in human blood. If I was to move forward with in-vivo testing in mice I would start with that max dose due to the positive results of bacteria inhibition at that concentration and then evaluate for any toxic effects.
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Do you believe the max dose would be applicable for all mammals?
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Do you think the max dose would be applicable for all mammals?
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Do you think the max does would be applicable for all mammals?
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Do you think the max dose would be applicable for all mammals?
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Sorry it commented so many times. My computer broke.
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