You mentioned that the low negative control marked your compound as not being a hit, but what does that low negative control indicate with your study? Isn’t the negative control used verify the accuracy of the test, and as such wouldn’t omitting it invalidate that analysis of your data?
the negative control was used to measure the absorbance recorded from the spectrophotometer, meaning a low negative control would mean the bacteria had died after being mixed with the water. I apologies I believe I might have forgotten to specify what the low value implied.
What about combining Mastoparan-X with the other compounds described in your future directions section would potentially make it more effective than Mastoparan-X by itself?
we were hoping to test to see if one of the compounds is more effective than the others and then could isolate the components of the compound which make it more effective
We used ampicillin because it is known to kill salmonella and we used water because it is known to not be harmful to salmonella as well our sample of mastoparan using water as its solvent
At what point in the lab were you able to deduce that the antibiotic was not a viable candidate for bacterial lysis? did everyone in your lab use different antibiotics?
We conducted a trial in which we incubated the bacteria for 24hrs after which we ran through a spectrophotometer. we then added samples from the wells to fresh bacteria, incubated for another 24 hrs and ran through a spectrophotometer again to determine if the bacteria were able to reproduce or if they were killed.
curesymposium.org 
You mentioned that the low negative control marked your compound as not being a hit, but what does that low negative control indicate with your study? Isn’t the negative control used verify the accuracy of the test, and as such wouldn’t omitting it invalidate that analysis of your data?
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the negative control was used to measure the absorbance recorded from the spectrophotometer, meaning a low negative control would mean the bacteria had died after being mixed with the water. I apologies I believe I might have forgotten to specify what the low value implied.
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Was there anything else you think stood out about your compound in order to test its antibacterial ability?
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Yes, our references mentioned that it works on both gram negative and gram positive bacteria.
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What about combining Mastoparan-X with the other compounds described in your future directions section would potentially make it more effective than Mastoparan-X by itself?
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we were hoping to test to see if one of the compounds is more effective than the others and then could isolate the components of the compound which make it more effective
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How did you determine what was going to be your positive and negative controls?
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We used ampicillin because it is known to kill salmonella and we used water because it is known to not be harmful to salmonella as well our sample of mastoparan using water as its solvent
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At what point in the lab were you able to deduce that the antibiotic was not a viable candidate for bacterial lysis? did everyone in your lab use different antibiotics?
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We conducted a trial in which we incubated the bacteria for 24hrs after which we ran through a spectrophotometer. we then added samples from the wells to fresh bacteria, incubated for another 24 hrs and ran through a spectrophotometer again to determine if the bacteria were able to reproduce or if they were killed.
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