In the second graph in the results section, what was the significance of using ampicillin and H2O as negative and positive controls/how can those results be interpreted?
A negative control and positive control are what we use to measure if the bacteria grew, died, or did nothing while in the incubation process. H20/DMSO was used as a negative control to see if the bacteria grew and was used to measure if we had a hit. While Ampicillin was used to measure if the bacteria died according to our results of the absorbance in our wells on the plate.
Research and development of genuinely new antibiotics are time-consuming and expensive and are often unsuccessful due to the growing rate of antibiotic-resistant bacteria.
The reason Antibiotics are so hard to find is because antibiotics that have been brought to market in the past three decades are variations of drugs already known. Research and development of new antibiotics are time-consuming and expensive and are often unsuccessful due to the growing rate of antibiotic-resistant bacteria.
You mentioned how Lentinan can potentially boost the immune system, but your trials focus on its abillity to directly impact the bacteria in question (without any immune system in your project). Do you think you would see different results if you used a live animal/human that has an immune system that can be boosted?
Before we are able to make the compounds way into humans or animals, we have to do the correct testing first to see the ability that the compound has and promising results in order to actually test in humans or animals because of funding and because of other limitations through clinical trials, we are just in the pre-clinical trials. In humans and animals, we could potentially see this depending on how the compound metabolizes so that the host’s immune system is boosted, also derived from our research lentinan was used in chemotherapies to actually boost host immune systems.
curesymposium.org 
In the second graph in the results section, what was the significance of using ampicillin and H2O as negative and positive controls/how can those results be interpreted?
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A negative control and positive control are what we use to measure if the bacteria grew, died, or did nothing while in the incubation process. H20/DMSO was used as a negative control to see if the bacteria grew and was used to measure if we had a hit. While Ampicillin was used to measure if the bacteria died according to our results of the absorbance in our wells on the plate.
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In the introduction you mentioned it’s been a while since any new classes of antibiotics have been found. Why is that?
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Research and development of genuinely new antibiotics are time-consuming and expensive and are often unsuccessful due to the growing rate of antibiotic-resistant bacteria.
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In this intro you mentioned it’s been a while since any new classes of antibiotics have been found. Why are they so hard to find?
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The reason Antibiotics are so hard to find is because antibiotics that have been brought to market in the past three decades are variations of drugs already known. Research and development of new antibiotics are time-consuming and expensive and are often unsuccessful due to the growing rate of antibiotic-resistant bacteria.
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You mentioned how Lentinan can potentially boost the immune system, but your trials focus on its abillity to directly impact the bacteria in question (without any immune system in your project). Do you think you would see different results if you used a live animal/human that has an immune system that can be boosted?
LikeLike
Before we are able to make the compounds way into humans or animals, we have to do the correct testing first to see the ability that the compound has and promising results in order to actually test in humans or animals because of funding and because of other limitations through clinical trials, we are just in the pre-clinical trials. In humans and animals, we could potentially see this depending on how the compound metabolizes so that the host’s immune system is boosted, also derived from our research lentinan was used in chemotherapies to actually boost host immune systems.
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