Given your result of no effect was consistent with previous different results, why would you continue diluting it down to find activity? What would indicate you may get different results at lower concentrations?
Hi! Actually, our data was not consistent with prior findings. VPA has been shown effective as a chemotherapeutic and an antibiotic for both Gram Positive and Gram Negative bacteria (as stated in our intro). Additionally, we saw a general trend that diluting VPA showed less absorbance (showing more antibiotic potential).
VPA acted as a chemotherapeutic by displaying histone deacetylase activity meaning the chromatin is more condensed and less genes are transcribed. This is relevant because a lot of antibiotics also work as chemotherapeutics (at higher doses), so we used that data as a jumping point to look if VPA had antibiotic potential.
It acts as a histone deacetylase meaning chromatin is more condensed and genes are less likely to be transcribed. Yes, it is relevant because many antibiotics act as a chemotherapeutic at high doses so we traced back from chemotherapeutic to antibiotic by diluting VPA.
Thank you! Our group was really interested in snake venom, but given how dangerous it is and how it expensive it is, we diced to go with valproic acid!
After listening to some of the Phage presentations it would be interesting to see if Phages treat Salmonella Typhimurium. Otherwise, antibiotics are a great way to treat Salmonella if the bacteria is not resistant to them.
curesymposium.org 
Given your result of no effect was consistent with previous different results, why would you continue diluting it down to find activity? What would indicate you may get different results at lower concentrations?
LikeLike
Hi! Actually, our data was not consistent with prior findings. VPA has been shown effective as a chemotherapeutic and an antibiotic for both Gram Positive and Gram Negative bacteria (as stated in our intro). Additionally, we saw a general trend that diluting VPA showed less absorbance (showing more antibiotic potential).
LikeLike
How did Valporic Acid act as a chemo drug and was it relevant to the study?
LikeLike
VPA acted as a chemotherapeutic by displaying histone deacetylase activity meaning the chromatin is more condensed and less genes are transcribed. This is relevant because a lot of antibiotics also work as chemotherapeutics (at higher doses), so we used that data as a jumping point to look if VPA had antibiotic potential.
LikeLike
It acts as a histone deacetylase meaning chromatin is more condensed and genes are less likely to be transcribed. Yes, it is relevant because many antibiotics act as a chemotherapeutic at high doses so we traced back from chemotherapeutic to antibiotic by diluting VPA.
LikeLike
Great presentation. What other substances would you want to test if you could?
LikeLike
Thank you! Our group was really interested in snake venom, but given how dangerous it is and how it expensive it is, we diced to go with valproic acid!
LikeLike
My question would be is there any other similar way to treat Salmaonella that you know of?
LikeLike
After listening to some of the Phage presentations it would be interesting to see if Phages treat Salmonella Typhimurium. Otherwise, antibiotics are a great way to treat Salmonella if the bacteria is not resistant to them.
LikeLike