Hi Chris! The max dose of any antibiotic permitted to be administered in humans is 10 uM. Above this threshold, it’s possible that Procyanidin B1 could effectively kill Salmonella Typhimurium at a level comparable to our positive control, ampicillin (far exceeding 2 SD below the mean absorbance of our negative control, 50% DMSO), but it would be highly toxic for other cells in the body. The exact value of the dose needed to meet this 2 SD requirement is not something I can predict without running more tests. Although, even if I were to find this perfect dose of Procyanidin B1, it wouldn’t be allowed for public use as it exceeds 10 uM. I hope this makes sense. Message me if you need more clarification!
Hi Chris! The max dose of any antibiotic allowed to be administered in humans is 10 uM. Above this threshold, it’s possible that Procyanidin B1 could effectively kill Salmonella Typhimurium at a level comparable to our positive control, ampicillin (far exceeding 2 SD below the mean absorbance of our negative control, 50% DMSO), but it would be highly toxic for other cells in the body. The exact value of the dose needed to meet this 2 SD requirement is not something I can predict without running more tests. Even if I were to find the perfect dose of Procyanidin B1, it wouldn’t be allowed for public use as it exceeds 10 uM. I hope this makes sense. Message me if you need more clarification!
Hi Kaylee! Procyanidin B1 (C30H26O12) is a compound we isolated from a fruit called Cornelian Cherries, which have historically been used to treat intestinal bacterial infections (through ingestion). They are apart of the Proanthocyanidin group.
Hi Logan! A probiotic could be used in an antibiotic study as a negative control (like 50% DMSO in this study). We compare the bacterial growth caused by potential antibiotics to the growth caused by a probiotic to determine whether it is a hit (2 SD above or below the mean of the negative control absorbance value).
We know this compound causes bacterial growth (using results from our dose-response and Mueller-Hinton plate tests). Typically, we want to see an antibiotic attack vital bacterial cell processes (including RNA/DNA synthesis, cell wall, cell membrane, folate synthesis, etc.). It’s possible that due to the compound’s molecular shape, it’s unable to interact productively with any of these processes.
Your graph shows that with a higher dosage of the compound comes a higher level of bacterial death, does this mean that it can sometimes be probiotic and sometimes have mild antibiotic properties?
This just means that the compound may have antibiotic properties at doses above 10 uM, the max dose allowed to be administered to humans in a clinical setting. If this compound was administered in a concentration lower than this, it would act as a probiotic.
curesymposium.org 
How high of a dose do you think you would need to get to that “hit” point you talked about of 2 standard deviations above/below the normal value?
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Hi Chris! The max dose of any antibiotic permitted to be administered in humans is 10 uM. Above this threshold, it’s possible that Procyanidin B1 could effectively kill Salmonella Typhimurium at a level comparable to our positive control, ampicillin (far exceeding 2 SD below the mean absorbance of our negative control, 50% DMSO), but it would be highly toxic for other cells in the body. The exact value of the dose needed to meet this 2 SD requirement is not something I can predict without running more tests. Although, even if I were to find this perfect dose of Procyanidin B1, it wouldn’t be allowed for public use as it exceeds 10 uM. I hope this makes sense. Message me if you need more clarification!
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Hi Chris! The max dose of any antibiotic allowed to be administered in humans is 10 uM. Above this threshold, it’s possible that Procyanidin B1 could effectively kill Salmonella Typhimurium at a level comparable to our positive control, ampicillin (far exceeding 2 SD below the mean absorbance of our negative control, 50% DMSO), but it would be highly toxic for other cells in the body. The exact value of the dose needed to meet this 2 SD requirement is not something I can predict without running more tests. Even if I were to find the perfect dose of Procyanidin B1, it wouldn’t be allowed for public use as it exceeds 10 uM. I hope this makes sense. Message me if you need more clarification!
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What is procyanidin B1?
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Hi Kaylee! Procyanidin B1 (C30H26O12) is a compound we isolated from a fruit called Cornelian Cherries, which have historically been used to treat intestinal bacterial infections (through ingestion). They are apart of the Proanthocyanidin group.
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If your compound turned out to be probiotic instead of antibiotic, in what way would it be effectively used in a lab?
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Hi Logan! A probiotic could be used in an antibiotic study as a negative control (like 50% DMSO in this study). We compare the bacterial growth caused by potential antibiotics to the growth caused by a probiotic to determine whether it is a hit (2 SD above or below the mean of the negative control absorbance value).
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What properties of procyanidin B1 do you think made it a better probiotic than antibiotic?
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We know this compound causes bacterial growth (using results from our dose-response and Mueller-Hinton plate tests). Typically, we want to see an antibiotic attack vital bacterial cell processes (including RNA/DNA synthesis, cell wall, cell membrane, folate synthesis, etc.). It’s possible that due to the compound’s molecular shape, it’s unable to interact productively with any of these processes.
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What properties of procyanidin B1 do you you think made it a better probiotic than antibiotic?
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Your graph shows that with a higher dosage of the compound comes a higher level of bacterial death, does this mean that it can sometimes be probiotic and sometimes have mild antibiotic properties?
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This just means that the compound may have antibiotic properties at doses above 10 uM, the max dose allowed to be administered to humans in a clinical setting. If this compound was administered in a concentration lower than this, it would act as a probiotic.
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