Absolutely! There still isn’t much research behind this but from the research we did get we focused on the link of tachystatins that correlates horseshoe crab and blue scorpion venom as an antimicrobial polypeptide. However there are multiple factors and peptides that could change the outcome. This is why for the future directions(if we can get our hands on horseshoe crab blood) we would like to preform the same tests we did on Vidatox!
Thank your for asking and sorry if it wasn’t clarified! That is our google draw drawing of our well plate. This is a plate where we mix our dilutions with the salmonella and incubate it following the control(figure 1.3). The well is then taken to spectrophotometer to read the absorption values to determine if the plate had any ‘hits’. If there are hits we can use the data from the plates to determine if it shows antibiotic properties or probiotic properties! Thanks to that we got some hits in the antibacterial range!
Do you believe that horseshoe crab blood and scorpion venom is a sustainable solution in comparison to possible solutions involving inanimate solutions?
Horseshoe crab blood and blue scorpion venom is definitely not a sustainable solution. Especially horseshoe crab blood, which is currently being used by a lot of studies to determine if a vaccine is good or not. These crabs are ancient and there aren’t many(this is why we couldn’t do an experiment on them due rarity and cost). So far I am not sure of any inanimate solutions sorry! I also don’t think is great for our microbiome, horseshoe crabs and scorpions play a role in the world, so taking them away in huge strains is hurting the environment unfortunately. If there is an inanimate solution or something that can be synthetically produced I would definitely hope we would use that instead!
Great question! To determine bactericidal or bacteriostatic we would first need a statistical hit on any of the dilutions and then do a separate experiment by putting the ‘hit’ dilutions in fresh media and see if the bacteria grows or not. If the bacteria grows then this is bacteriostatic but if the bacteria decreases then it is bactericidal. We would of course do multiple trials to make sure the data correlates with each other. We would use the same controls and test our negative and positive controls with the salmonella to make sure we have a reference. We were out of time to do such a test so we hope future students can use our data and go ahead with the experiment!
curesymposium.org 
Could the differences between actual horseshoe crab blood and the Vidatox result in a different outcome?
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Absolutely! There still isn’t much research behind this but from the research we did get we focused on the link of tachystatins that correlates horseshoe crab and blue scorpion venom as an antimicrobial polypeptide. However there are multiple factors and peptides that could change the outcome. This is why for the future directions(if we can get our hands on horseshoe crab blood) we would like to preform the same tests we did on Vidatox!
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What is figure 2 under methods showing or how does it relate to your experiment?
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Thank your for asking and sorry if it wasn’t clarified! That is our google draw drawing of our well plate. This is a plate where we mix our dilutions with the salmonella and incubate it following the control(figure 1.3). The well is then taken to spectrophotometer to read the absorption values to determine if the plate had any ‘hits’. If there are hits we can use the data from the plates to determine if it shows antibiotic properties or probiotic properties! Thanks to that we got some hits in the antibacterial range!
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Do you believe that horseshoe crab blood and scorpion venom is a sustainable solution in comparison to possible solutions involving inanimate solutions?
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Horseshoe crab blood and blue scorpion venom is definitely not a sustainable solution. Especially horseshoe crab blood, which is currently being used by a lot of studies to determine if a vaccine is good or not. These crabs are ancient and there aren’t many(this is why we couldn’t do an experiment on them due rarity and cost). So far I am not sure of any inanimate solutions sorry! I also don’t think is great for our microbiome, horseshoe crabs and scorpions play a role in the world, so taking them away in huge strains is hurting the environment unfortunately. If there is an inanimate solution or something that can be synthetically produced I would definitely hope we would use that instead!
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How would you describe the processes for future directions to determine whether it is bactericidal or bacteriostatic?
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Great question! To determine bactericidal or bacteriostatic we would first need a statistical hit on any of the dilutions and then do a separate experiment by putting the ‘hit’ dilutions in fresh media and see if the bacteria grows or not. If the bacteria grows then this is bacteriostatic but if the bacteria decreases then it is bactericidal. We would of course do multiple trials to make sure the data correlates with each other. We would use the same controls and test our negative and positive controls with the salmonella to make sure we have a reference. We were out of time to do such a test so we hope future students can use our data and go ahead with the experiment!
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Great job Shriya! It was nice working with you this semester on this research!
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you to madison!!!!
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