So we used DMSO as the negative control and Ampicillin as the positive control. DMSO was used cause it’s what compounds are dissolved in should ideally have no effect on the bacteria. Ampicillin was used because it’s known to kill the bacteria so we have something to compare our potential antibiotic compound too.
It’s funny you ask that, originally we wanted to look at tea tree oil but that had actually already been researched and apparently wasn’t very promising.
A catechin is the name for a specific species of antioxidant that’s present in green tea. We decided to look at it cause antioxidants are already known for their cancer prevention capablities. The particular one we researched, ECGC, had previously been looked at in a large, general study and they found that it showed potential in killing salmonella colonies.
I can’t fully answer this as this is still one of my biggest questions finishing this research. I do know that according to Dr.Harvey many organic compounds seem to show a bell curve in effectiveness when tested at different concentrations. While our data wasn’t a bell curve the general shape does show a resemblance to this concept.
The 8% had a greater absorbency because there was a greater amount of active salmonella in those wells. 4% and 12% had lower absorbencies because they reduced bacterial activity. As for why this happens at great and lower concentrations I unfortunately cannot say.
A catechin is a kind of antioxidant that’s found in green tea, if I were to guess its purpose in the plant itself it probably aids in photosynthesis like carotenes do. Yes, it was cool to see everyone’s different compounds. Somebody at my table was actually studying camels milk, that was interesting to see for sure.
Table 2 shows how when the salmonella sample was removed from the well with the ECGC it began to grow. This means that the compound was preventing growth and when it was no longer in the equation the bacteria began to grow again.
curesymposium.org 
Hi! How did you decide what to use for your positive and negative controls?
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So we used DMSO as the negative control and Ampicillin as the positive control. DMSO was used cause it’s what compounds are dissolved in should ideally have no effect on the bacteria. Ampicillin was used because it’s known to kill the bacteria so we have something to compare our potential antibiotic compound too.
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Hi Cole! I was wondering if this research with salmonella has been done with other types of teas or herbs?
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It’s funny you ask that, originally we wanted to look at tea tree oil but that had actually already been researched and apparently wasn’t very promising.
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Hello! What exactly is a Catechin and how did you decide to use it?
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A catechin is the name for a specific species of antioxidant that’s present in green tea. We decided to look at it cause antioxidants are already known for their cancer prevention capablities. The particular one we researched, ECGC, had previously been looked at in a large, general study and they found that it showed potential in killing salmonella colonies.
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HI, great presentation. Why do you think think that the 8% concentration had the greatest absorbency compared to the extremes of 12% and 4%?
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I can’t fully answer this as this is still one of my biggest questions finishing this research. I do know that according to Dr.Harvey many organic compounds seem to show a bell curve in effectiveness when tested at different concentrations. While our data wasn’t a bell curve the general shape does show a resemblance to this concept.
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hi, great presentation. I was wondering why the 8% dose had a greater absorbency than either of the extremes (4% and 12% doses) as shown in Graph 1?
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The 8% had a greater absorbency because there was a greater amount of active salmonella in those wells. 4% and 12% had lower absorbencies because they reduced bacterial activity. As for why this happens at great and lower concentrations I unfortunately cannot say.
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i was wondering what exactly a Catechin is within green tea, and did everyone in your lab use different potential antibiotics?
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A catechin is a kind of antioxidant that’s found in green tea, if I were to guess its purpose in the plant itself it probably aids in photosynthesis like carotenes do. Yes, it was cool to see everyone’s different compounds. Somebody at my table was actually studying camels milk, that was interesting to see for sure.
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Hi, could you quickly explain what table 2 means?
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Table 2 shows how when the salmonella sample was removed from the well with the ECGC it began to grow. This means that the compound was preventing growth and when it was no longer in the equation the bacteria began to grow again.
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