Nice job! Cool results. Given your results that Betulin is bacteriostatic, would you predict it would be best used against any bacterial infection or specifically against multi-drug resistant bacteria?
Depends on whether we have a new MoA for antibiotic activity that hasn’t been discovered or widely used yet. Hopefully it is a totally new mechanism that could be used against a multi-drug resistant strain otherwise and probably more likely it is just a new compound with an old mechanism and then it would not be effective against the MDR strains.
If you had started with 10uM and got the wanted results and could, would you have continued to experiment with higher doses to see their effects or if they were more/less effective?
We wouldn’t have experimented with any dose higher than 10uM because that would be the highest achievable dosage in the bloodstream, so any results at a higher dose wouldn’t be applicable.
Very informative and interesting presentation. You stated that you picked Betulin because due to prior studies it showed that it has antibiotic activity and has been used in the past to heal wounds for example. Do you believe we should use this more often to heal wounds similar to its uses in the past because of its wide range of benefits and uses?
If you were successful on getting the 10uM dose the first time, would you, if allowed, experiment further to see the effects/effectiveness of higher doses?
When my group was researching which compound to choose we came across this information,
“In studies done by Liu et al., the antimicrobial properties of betulin were examined with several microbes including “Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Fusarium graminearum, Gibberella zeae, and Cercosporella albo-maculans” (Liu et al.)”
So one of the key reasons we chose this compound is that previous studies had determined betulin does have antibiotic activity with several types of bacterium. Yes, there is evidence that betulin would be effective against several types of infections!
I have no information on side effects of betulin because we haven’t been able to move on to in vivo studies which would allow us to see any potential side effects.
curesymposium.org 
Nice job! Cool results. Given your results that Betulin is bacteriostatic, would you predict it would be best used against any bacterial infection or specifically against multi-drug resistant bacteria?
LikeLike
Depends on whether we have a new MoA for antibiotic activity that hasn’t been discovered or widely used yet. Hopefully it is a totally new mechanism that could be used against a multi-drug resistant strain otherwise and probably more likely it is just a new compound with an old mechanism and then it would not be effective against the MDR strains.
LikeLike
If you had started with 10uM and got the wanted results and could, would you have continued to experiment with higher doses to see their effects or if they were more/less effective?
LikeLike
We wouldn’t have experimented with any dose higher than 10uM because that would be the highest achievable dosage in the bloodstream, so any results at a higher dose wouldn’t be applicable.
LikeLike
Very informative and interesting presentation. You stated that you picked Betulin because due to prior studies it showed that it has antibiotic activity and has been used in the past to heal wounds for example. Do you believe we should use this more often to heal wounds similar to its uses in the past because of its wide range of benefits and uses?
LikeLike
Betulin could be useful as an ingredient in a salve to prevent bacterial growth because it exhibited bacteriostatic activity in our studies.
LikeLike
If you were successful on getting the 10uM dose the first time, would you, if allowed, experiment further to see the effects/effectiveness of higher doses?
LikeLike
sorry – ignore this, the first time I commented it wasn’t showing.
LikeLike
Great job! Do you think Betulin could be used in other bacterial infections and not just what you tested?
LikeLike
Good job! Do you think Betulin could be used on other bacterial infections or just the one you tested?
LikeLike
When my group was researching which compound to choose we came across this information,
“In studies done by Liu et al., the antimicrobial properties of betulin were examined with several microbes including “Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Fusarium graminearum, Gibberella zeae, and Cercosporella albo-maculans” (Liu et al.)”
So one of the key reasons we chose this compound is that previous studies had determined betulin does have antibiotic activity with several types of bacterium. Yes, there is evidence that betulin would be effective against several types of infections!
LikeLike
Are there any negative side effects of betulin that could potentially harm a cancer patient?
LikeLike
I have no information on side effects of betulin because we haven’t been able to move on to in vivo studies which would allow us to see any potential side effects.
LikeLike