Hi Geneva! We ended up falling behind a bit because our ink got vortexed by another individual in the lab before we were able to separate it, making us have to wait an additional 2 days for it to settle and separate. Because of this, we took out data from the dose response curve and applied it to the Max Dose. In our top graph, you can see the very left bar is 100% concentration and the next bar 10%, so this data shows the same thing the Max Dose procedure would, it was just all included into one plate!
Great Job Rachel! Are there kinds of bacteria similar to salmonella typhimurium that are more harmful to humans like in the case of typhoid fever in mice that this could be used to treat?
Yes! Salmonella Typhi- which causes typhoid fever in humans- would be a great example of this. S. Typhi is much more deadly to humans but has a nearly identical structure and mechanism to that of S. Typhimurium. Because of this similarity, we can assume that if cuttlefish ink acts as a bacteriostatic compound against S. Typhimurium, it will also inhibit the growth of S. Typhi and treat Typhoid Fever.
Thank you Brielle! It is hard to determine whether or not Trial 3 would have had a negative impact on our results because the negative control was invalid. Without a negative control, we did not have a standard for comparison to see how much the bacteria did or did not grow. It is not that we got bad results and decided not to include them, it is that the trial 3 results are not interpretable. The lack of negative control indicates human error in the protocol, not a shortcoming of the compound. Including it in our results would be difficult because there is no way to determine a hit or not without a negative mean/standard deviations to guide our analysis.
Hi Simran, I think it would depend. As I am currently unsure of the mechanisms the compounds within cuttlefish ink extract use, this is hard to determine. I would say if the structure and mechanism of the stronger bacteria is similar to S. Typhi/Typhimurium, it is very possible! I would expect the bacteria to be gram negative and rod shaped.
curesymposium.org 
Great job Rachel! Was your group able to run a Max Dose experiment? If so, what were the results?
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Hi Geneva! We ended up falling behind a bit because our ink got vortexed by another individual in the lab before we were able to separate it, making us have to wait an additional 2 days for it to settle and separate. Because of this, we took out data from the dose response curve and applied it to the Max Dose. In our top graph, you can see the very left bar is 100% concentration and the next bar 10%, so this data shows the same thing the Max Dose procedure would, it was just all included into one plate!
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Great Job Rachel! Are there kinds of bacteria similar to salmonella typhimurium that are more harmful to humans like in the case of typhoid fever in mice that this could be used to treat?
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Yes! Salmonella Typhi- which causes typhoid fever in humans- would be a great example of this. S. Typhi is much more deadly to humans but has a nearly identical structure and mechanism to that of S. Typhimurium. Because of this similarity, we can assume that if cuttlefish ink acts as a bacteriostatic compound against S. Typhimurium, it will also inhibit the growth of S. Typhi and treat Typhoid Fever.
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Great job presenting Rachel! Do you think that if trial 3 was not omitted it would have been a success? If so, how would this change your conclusion?
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Thank you Brielle! It is hard to determine whether or not Trial 3 would have had a negative impact on our results because the negative control was invalid. Without a negative control, we did not have a standard for comparison to see how much the bacteria did or did not grow. It is not that we got bad results and decided not to include them, it is that the trial 3 results are not interpretable. The lack of negative control indicates human error in the protocol, not a shortcoming of the compound. Including it in our results would be difficult because there is no way to determine a hit or not without a negative mean/standard deviations to guide our analysis.
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Hi!! Can this treatment/method be used on bacteria stronger than S. Typhi?
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Hi Simran, I think it would depend. As I am currently unsure of the mechanisms the compounds within cuttlefish ink extract use, this is hard to determine. I would say if the structure and mechanism of the stronger bacteria is similar to S. Typhi/Typhimurium, it is very possible! I would expect the bacteria to be gram negative and rod shaped.
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