What exactly does the term bactericidal and bacteriostatic? Why does the above line in figure 2 I believe it is, as the plus 2 standard deviations of the negative control imply that it is a potential probiotic? What makes this the case?
Bactericidal antibiotics are antibiotics that kill bacteria while bacteriostatic antibiotics only stop the growth of bacteria. Even though bacteriostatic antibiotics don’t kill bacteria, they are still effective antibiotics because they give the body the time they need to get rid of the bacterial infection. The negative control is used because it is a compound that is known to have no effect on bacteria, so if there is a result outside of two standard deviations, you can be about 95% sure that the compound you are testing is either a potential antibiotic or a potential probiotic. I hope that answered your questions.
Honestly, I’m not sure if testing a higher concentration would’ve showed a different result. My guess is that it would kill more bacteria but we had some mixed results so I can’t say what would happen for certain. We definitely would’ve liked to test a higher concentration but 10% is the highest concentration that the human bloodstream can absorb, so testing any concentration that is higher than 10% would’ve just been a waste of time.
Unfortunately, a 10% concentration is the concentration that the human bloodstream is able to absorb so there is no point in testing a higher concentration. The reason we would want to test Alpha Pinene in vivo is because sometimes the interactions in a living organism will provide different results than just testing the compound directly against Salmonella Typhimurium. Honestly, we probably wouldn’t find any different results but we think it is still worth trying just to see what would happen.
curesymposium.org 
What exactly does the term bactericidal and bacteriostatic? Why does the above line in figure 2 I believe it is, as the plus 2 standard deviations of the negative control imply that it is a potential probiotic? What makes this the case?
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Bactericidal antibiotics are antibiotics that kill bacteria while bacteriostatic antibiotics only stop the growth of bacteria. Even though bacteriostatic antibiotics don’t kill bacteria, they are still effective antibiotics because they give the body the time they need to get rid of the bacterial infection. The negative control is used because it is a compound that is known to have no effect on bacteria, so if there is a result outside of two standard deviations, you can be about 95% sure that the compound you are testing is either a potential antibiotic or a potential probiotic. I hope that answered your questions.
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Do you think your results would have been different with a higher dose of Alpha-Pinene? If not what do you think would have an effect?
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Honestly, I’m not sure if testing a higher concentration would’ve showed a different result. My guess is that it would kill more bacteria but we had some mixed results so I can’t say what would happen for certain. We definitely would’ve liked to test a higher concentration but 10% is the highest concentration that the human bloodstream can absorb, so testing any concentration that is higher than 10% would’ve just been a waste of time.
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Do you think a higher does test would be better before heading to in vivo tests? I think having a proof of concept is needed before in vivo testing.
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Unfortunately, a 10% concentration is the concentration that the human bloodstream is able to absorb so there is no point in testing a higher concentration. The reason we would want to test Alpha Pinene in vivo is because sometimes the interactions in a living organism will provide different results than just testing the compound directly against Salmonella Typhimurium. Honestly, we probably wouldn’t find any different results but we think it is still worth trying just to see what would happen.
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