I am not sure to be completely honest. It is safer to assume that Mrycene would have some effect on other strains of Salmonella due to Mrycene already having some effect on S. Typhimurium. That being said, Mrycene has been shown to cross link DNA which should have an effect in all organisms, so it might be helpful in fighting other type of bacteria, but it is uncertain unless tested.
A hit range is a range determined by the average DMSO absorbance (negative control) minus two standard deviations of the DMSO sample. The negative control has no effect on salmonella growth, so the hit range is looking for wells that show statistically significant amounts of growth/lack thereof compared to normal salmonella growth. When I say that our group had some hits that means the absorbance of that concentration of Myrcene has a lack of growth that wouldn’t be normal otherwise, showing that Myrcene is definitively preventing salmonella growth at that certain concentration.
This was a good presentation, super well put together. How could this possibly be administered as an antibiotic? What infections could it possibly target?
Thank you! To be honest though I am unsure of the best route of administration. If possible it would be cheaper to have the Mrycene made into an oral pill than having to be injected by IV. If the IV method is used though it would avoid the headache of dealing with all the enzymes the Mrycene would meet when it travels through the mouth, into the stomach, and finally into the bloodstream. Also, as of right now the only bacterial infection that Mrycene can fight against is S. Typhimurium, and even then our groups data is somewhat inconclusive. It is possible that Myrcene has an effect on other strain of Salmonella but it is too early to tell whether it will have an effect of other bacterial infections.
Great job explaining your methods. Do you belive Myrcene could be helpful at fighting aginst other types of bacterial infections, not just Salmonella?
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I am not sure to be completely honest. It is safer to assume that Mrycene would have some effect on other strains of Salmonella due to Mrycene already having some effect on S. Typhimurium. That being said, Mrycene has been shown to cross link DNA which should have an effect in all organisms, so it might be helpful in fighting other type of bacteria, but it is uncertain unless tested.
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You mention hit and hit range quite often, what is the implication of these terms?
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A hit range is a range determined by the average DMSO absorbance (negative control) minus two standard deviations of the DMSO sample. The negative control has no effect on salmonella growth, so the hit range is looking for wells that show statistically significant amounts of growth/lack thereof compared to normal salmonella growth. When I say that our group had some hits that means the absorbance of that concentration of Myrcene has a lack of growth that wouldn’t be normal otherwise, showing that Myrcene is definitively preventing salmonella growth at that certain concentration.
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This was a good presentation, super well put together. How could this possibly be administered as an antibiotic? What infections could it possibly target?
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Thank you! To be honest though I am unsure of the best route of administration. If possible it would be cheaper to have the Mrycene made into an oral pill than having to be injected by IV. If the IV method is used though it would avoid the headache of dealing with all the enzymes the Mrycene would meet when it travels through the mouth, into the stomach, and finally into the bloodstream. Also, as of right now the only bacterial infection that Mrycene can fight against is S. Typhimurium, and even then our groups data is somewhat inconclusive. It is possible that Myrcene has an effect on other strain of Salmonella but it is too early to tell whether it will have an effect of other bacterial infections.
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