You said that higher concentrations of Cinnamic acid showed a significant inhibition of bacterial growth. What are the initial concentrations of Cinnamic acid that you used and why did you choose to experiment on these concentrations and not the higher concentrations?
We used 10um (micromolars), 5um, 2.5um and 1um are the initial concentrations. These were the initial concentrations that we used, but none of them inhibited bacterial growth. But when we used 25um and above, and we did experiment it, it led to significant death in the bacteria. The reason we didn’t present that data is because that concentration would have been too high in human blood, so it would’t have been physiologically relevant data.
A hit is defined as anything that is plus or minus two standard deviations of the mean of the negative control, which in our case was DMSO. Basically, if the absorbance of the compound was above two standard deviations, it would mean that the bacteria grew, and if it was below two standard deviations, it would mean the bacteria died. The hit range just basically means that if it is outside of the two standard deviations it is within the range of being a hit.
I would guess that because we used concentrations of Cinnamic Acid that are physiologically relevant and it led to bacterial growth that if an animal had a bacterial infection and we used Cinnamic Acid on the animal, it would die from the infection.
Is there a way to create the higher concentrations necessary for action as an antibiotic in a manor where they are directed to the infection without increasing the risk of toxicity for the patient?
Honestly, I am not sure but if there was a way to utilize it in such a way that it could be an antibiotic, but that would definitely be ideal. Good question!
curesymposium.org 
You said that higher concentrations of Cinnamic acid showed a significant inhibition of bacterial growth. What are the initial concentrations of Cinnamic acid that you used and why did you choose to experiment on these concentrations and not the higher concentrations?
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We used 10um (micromolars), 5um, 2.5um and 1um are the initial concentrations. These were the initial concentrations that we used, but none of them inhibited bacterial growth. But when we used 25um and above, and we did experiment it, it led to significant death in the bacteria. The reason we didn’t present that data is because that concentration would have been too high in human blood, so it would’t have been physiologically relevant data.
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Is there a difference between Hit and Hits range?
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A hit is defined as anything that is plus or minus two standard deviations of the mean of the negative control, which in our case was DMSO. Basically, if the absorbance of the compound was above two standard deviations, it would mean that the bacteria grew, and if it was below two standard deviations, it would mean the bacteria died. The hit range just basically means that if it is outside of the two standard deviations it is within the range of being a hit.
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Is there a difference between hit and htis range?
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I know that you said it was invitro, but do you have any sort of hypothesis on how it would’ve affected a living animal?
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I would guess that because we used concentrations of Cinnamic Acid that are physiologically relevant and it led to bacterial growth that if an animal had a bacterial infection and we used Cinnamic Acid on the animal, it would die from the infection.
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Is there a way to create the higher concentrations necessary for action as an antibiotic in a manor where they are directed to the infection without increasing the risk of toxicity for the patient?
LikeLike
Honestly, I am not sure but if there was a way to utilize it in such a way that it could be an antibiotic, but that would definitely be ideal. Good question!
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