For the experiments, how are the positive and negative controls chosen and what do they add to the overall understanding of the results of the experiments?
The positive control Ampicillin is a known bacteria which means we can compare our results to it, and if the compound was effective it would yield results similar to Ampicillin. The negative control DMSO was chosen because it has no effect on the bacteria and Camphor was dissolved in it. This means any effects that the DMSO had would be accounted for.
When a drug enters the body it has to go through many systems before it reaches its target and typically it is difficult to achieve a concentration higher than 10ug/mL in the blood. Additionally, too much of any drug has the potential for toxicities.
I imagine that these compounds are found at much higher concentrations in the plants they originate from in order to be as effective as they seem in nature. Do you know what the concentrations of these compounds would be in a normal plant?
Camphor was shown to have different effects based on where it was derived from in the Camphor tree in our research. We could try different types of Camphor in a similar way to see if they would be more effective. Combining it with other compounds like you said could also be potentially effective.
Going forward, how would you make the camphor more effective, similar to the first dose? Do you think it would be possible, and do you have any particular ideas?
For the experiments, how are the positive and negative controls chosen and what do they add to the overall understanding of the results of the experiments?
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The positive control Ampicillin is a known bacteria which means we can compare our results to it, and if the compound was effective it would yield results similar to Ampicillin. The negative control DMSO was chosen because it has no effect on the bacteria and Camphor was dissolved in it. This means any effects that the DMSO had would be accounted for.
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The max concentration in the human blood was mentioned, what would happen if the max concentration was exceeded.
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When a drug enters the body it has to go through many systems before it reaches its target and typically it is difficult to achieve a concentration higher than 10ug/mL in the blood. Additionally, too much of any drug has the potential for toxicities.
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I imagine that these compounds are found at much higher concentrations in the plants they originate from in order to be as effective as they seem in nature. Do you know what the concentrations of these compounds would be in a normal plant?
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I am not sure, but I think when Camphor is first distilled from the Camphor tree it is in its pure state.
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How is Camphor isolated from all of the other compounds found in lavender oil?
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Camphor is a part of lavender oil but not derived from it. We used Camphor from the wood of the Camphor tree.
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Is there a way to surpass the maximum dose to make camphor more effective?
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We could test a higher dose using the same lab techniques. However, I don’t think this would be as achievable in a clinical or real life setting.
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Do you have any idea how camphor could be made more effective? For example, could the dose be increased or could it be combined with another compound?
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Camphor was shown to have different effects based on where it was derived from in the Camphor tree in our research. We could try different types of Camphor in a similar way to see if they would be more effective. Combining it with other compounds like you said could also be potentially effective.
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Going forward, how would you make the camphor more effective, similar to the first dose? Do you think it would be possible, and do you have any particular ideas?
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