Why do you think that the 10 mg vs. 1 mg of silver nanoparticles had such drastically different effects on the absorbance readings – you mention them being a growth promoter but why does it change at a lower concentration?
The 10mg of silver particles had higher absorbance readings likely because there was a greater concentration of particles themselves. This seemed to change at a lower concentration because there were less particles being picked up by the spectrophotometer due to there being less particles and also because the Salmonella growth seemed to have been inhibited by the particles. We cannot definitively say whether our compound was killing the bacteria or not because we did not receive results for a bactericidal/bacteriostatic test though so it’s hard to say.
What do you think could be some limitations or risks for using silver nanoparticles to treat infections in humans? If it may act as an endocrine disrupter as mentioned, would this make it unsafe to use in the treatment of living organisms?
Yes, and these compounds have not been tested very much in vitro yet, but the next step would be to test it in animals such as rats to test the effects in a body. But because they penetrate cells, it could be potentially risky for other cells that aren’t the targeted bacterial cells.
In short, a bacteriostatic/bactericidal test tells you if your compound kills bacteria or if it promotes the growth of bacteria. You would run a plate with your compound and bacteria through a spectrophotometer and wait 24 hours, then run the plate again to see if there is a change in absorbance values. If there has been any sign of growth or death, the wells with the growth or death are then put into a new media for another 24 hours and the results of the well plate after the full 48 hour period will hopefully give you the results of whether your compound is bacteria killing or bacteria growth promoting (potential antibiotic or probiotic).
curesymposium.org 
Why do you think that the 10 mg vs. 1 mg of silver nanoparticles had such drastically different effects on the absorbance readings – you mention them being a growth promoter but why does it change at a lower concentration?
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The 10mg of silver particles had higher absorbance readings likely because there was a greater concentration of particles themselves. This seemed to change at a lower concentration because there were less particles being picked up by the spectrophotometer due to there being less particles and also because the Salmonella growth seemed to have been inhibited by the particles. We cannot definitively say whether our compound was killing the bacteria or not because we did not receive results for a bactericidal/bacteriostatic test though so it’s hard to say.
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What do you think could be some limitations or risks for using silver nanoparticles to treat infections in humans? If it may act as an endocrine disrupter as mentioned, would this make it unsafe to use in the treatment of living organisms?
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Yes, and these compounds have not been tested very much in vitro yet, but the next step would be to test it in animals such as rats to test the effects in a body. But because they penetrate cells, it could be potentially risky for other cells that aren’t the targeted bacterial cells.
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Great presentation -how would a conducting a bactericidal/bacteriostatic test see if compound inhibits growth or kill salmonella typhimurium?
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In short, a bacteriostatic/bactericidal test tells you if your compound kills bacteria or if it promotes the growth of bacteria. You would run a plate with your compound and bacteria through a spectrophotometer and wait 24 hours, then run the plate again to see if there is a change in absorbance values. If there has been any sign of growth or death, the wells with the growth or death are then put into a new media for another 24 hours and the results of the well plate after the full 48 hour period will hopefully give you the results of whether your compound is bacteria killing or bacteria growth promoting (potential antibiotic or probiotic).
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Why do you think it changed so dramatically after diluting it?
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